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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Overview
Product name
Anti-OBFC2B antibody
See all OBFC2B products (3) ...
Description
Mouse polyclonal to OBFC2B
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length (aa 1-211) human OBFC2B (NP_076973).
Positive control
OBFC2B transfected 293T lysate; HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
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Western blot - OBFC2B antibody (ab68812)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab68812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 30 kDa (predicted molecular weight: 23 kDa).
ICC/IF: Use at an assay dependent concentration. (Requires purification prior to ICC/IF.)
Target
Relevance
Single-stranded DNA (ssDNA)-binding proteins, such as OBFC2B, are ubiquitous and essential for a variety of DNA metabolic processes, including replication, recombination, and detection and repair of damage. OBFC2B plays an important role in DNA damage response. It influences diverse endpoints in the cellular DNA damage response, including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. It associates with DNA lesions, and enables the efficient activation of ATM and consequent phosphorylation of downstream proteins.
Cellular localization
Nuclear. Localizes to nuclear foci following DNA damage.
Alternative names
- MGC2731 antibody
- Nabp2 antibody
- SOSS complex subunit B1 antibody
see all
Database links
- Entrez Gene: 79035 Human
- Omim: 612104 Human
- SwissProt: Q9BQ15 Human
Anti-OBFC2B antibody images:
Western blot - OBFC2B antibody (ab68812)
All lanes : Anti-OBFC2B antibody (ab68812) at 1/500 dilution
Lane 1 : OBFC2B transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 23 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
References for Anti-OBFC2B antibody (ab68812)
ab68812 has not yet been referenced specifically in any publications.
Publishing research using ab68812? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"