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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Overview
Product name
Anti-OBFC2B antibody
See all OBFC2B products (3) ...
Description
Rabbit polyclonal to OBFC2B
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide, corresponding to a region between residues 161 and 211 of human OBFC2B (NP_076973.1)
Positive control
HeLa and 293T whole cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Applications
Show applications key
Our Abpromise guarantee covers the use of ab85752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000.Detects a band of approximately 33 kDa (predicted molecular weight: 23 kDa).
IP: Use at 2-5 µg/mg of lysate.
Target
Relevance
Single-stranded DNA (ssDNA)-binding proteins, such as OBFC2B, are ubiquitous and essential for a variety of DNA metabolic processes, including replication, recombination, and detection and repair of damage. OBFC2B plays an important role in DNA damage response. It influences diverse endpoints in the cellular DNA damage response, including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. It associates with DNA lesions, and enables the efficient activation of ATM and consequent phosphorylation of downstream proteins.
Cellular localization
Nuclear. Localizes to nuclear foci following DNA damage.
Alternative names
- MGC2731 antibody
- Nabp2 antibody
- SOSS complex subunit B1 antibody
see all
Database links
- Entrez Gene: 79035 Human
- Omim: 612104 Human
- SwissProt: Q9BQ15 Human
Anti-OBFC2B antibody images:
Western blot - OBFC2B antibody (ab85752)
All lanes : Anti-OBFC2B antibody (ab85752) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size : 23 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
Immunoprecipitation - OBFC2B antibody (ab85752)
Detection of Human OBFC2B by Western Blot of Immunprecipitate.
All lanes: ab85752 at 1µg/ml detecting by WB OBFC2B in HeLa whole cell lysates, that were immunoprecipitated with ab85752 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 1: ab85752 Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 5 seconds.
References for Anti-OBFC2B antibody (ab85752)
ab85752 has not yet been referenced specifically in any publications.
Publishing research using ab85752? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"