|
|
|
|
Western blot - OBFC2B antibody (ab85752)
(enlarge)
|
|
Immunoprecipitation - OBFC2B antibody (ab85752)
(enlarge)
|
|
|
|
|
Product Name
|
OBFC2B antibody
|
| |
See all OBFC2B antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to OBFC2B
|
|
Immunogen
|
Synthetic peptide, corresponding to a region between residues 161 and 211 of human OBFC2B (NP_076973.1)
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
HeLa and 293T whole cell lysates.
|
|
Cellular localization
|
Nuclear. Localizes to nuclear foci following DNA damage.
|
|
Research areas
|
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
|
|
Relevance
|
Single-stranded DNA (ssDNA)-binding proteins, such as OBFC2B, are ubiquitous and essential for a variety of DNA metabolic processes, including replication, recombination, and detection and repair of damage.
OBFC2B plays an important role in DNA damage response. It influences diverse endpoints in the cellular DNA damage response, including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. It associates with DNA lesions, and enables the efficient activation of ATM and consequent phosphorylation of downstream proteins.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this OBFC2B antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for OBFC2B antibody images, references, products related to ab85752 and other tools.
|
| |
OBFC2B antibody images:
|
 Western blot - OBFC2B antibody (ab85752)
All lanes : OBFC2B antibody (ab85752) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size : 23 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
|
| |
Immunoprecipitation - OBFC2B antibody (ab85752)
Detection of Human OBFC2B by Western Blot of Immunprecipitate.
All lanes: ab85752 at 1µg/ml detecting by WB OBFC2B in HeLa whole cell lysates, that were immunoprecipitated with ab85752 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 1: ab85752 Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 5 seconds.
|
| |
| |
|
Search PubMed (MEDLINE) for references to OBFC2B
|
| |
OBFC2B antibody - more information
|
Customer reviews (feedback) regarding OBFC2B antibody |
Customer FAQs regarding OBFC2B antibody |
Protocols for OBFC2B antibody |
Price and availability of products related to OBFC2B antibody |
| |
OBFC2B antibody - related products:
|
|
|
| |
OBFC2B antibody - other tools:
|
Contact Abcam with a Technical Enquiry about OBFC2B antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
