Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab106319)
Key features and details
- Rabbit polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody
See all OGT / O-Linked N-Acetylglucosamine Transferase primary antibodies -
Description
Rabbit polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Gorilla, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide corresponding to Human OGT/ O-Linked N-Acetylglucosamine Transferase aa 500-600 conjugated to keyhole limpet haemocyanin.
(Peptide available asab129221) -
Positive control
- This antibody gave a positive signal in both HeLa and A549 whole cell lysates and HepG2 cell line.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).
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ICC/IF |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
Target
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Function
Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. -
Tissue specificity
Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the O-GlcNAc transferase family.
Contains 13 TPR repeats. -
Domain
The TPR repeat domain mediates recognition of protein substrates. -
Post-translational
modificationsUbiquitinated, leading to its proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. Mostly in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 532053 Cow
- Entrez Gene: 8473 Human
- Entrez Gene: 108155 Mouse
- Entrez Gene: 664652 Pig
- Entrez Gene: 100354727 Rabbit
- Entrez Gene: 26295 Rat
- Omim: 300255 Human
- SwissProt: O15294 Human
see all -
Alternative names
- FLJ23071 antibody
- GlcNAc transferase antibody
- HRNT1 antibody
see all
Images
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All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab106319) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 116 kDa
Observed band size: 116 kDa
Additional bands at: 170 kDa, 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
ICC/IF image of ab106319 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106319, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) MCF7 cells at 5µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab106319 has not yet been referenced specifically in any publications.