Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cancer >> Tumor biomarkers >> Other
Overview
Product name
Anti-OLFM4 antibody
See all OLFM4 products (5) ...
Description
Rabbit polyclonal to OLFM4
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Cow
Immunogen
A region within a synthetic peptide: AFMVCGVLYA TRTMNTRTEE IFYYYDTNTG KEGKLDIVMH KMQEKVQSIN, corresponding to C terminal amino acids 433-482 of Human OLFM4
AFMVCGVLYA TRTMNTRTEE IFYYYDTNTG KEGKLDIVMH KMQEKVQSIN
Positive control
Jurkat cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
-
Western blot - OLFM4 antibody (ab65443)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 5 µg/mlDetects a band of approximately 50 kDa (predicted molecular weight: 57 kDa).
Target
Function
An antiapoptotic factor that promotes tumor growth. Promotes proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. Facilitates cell adhesion.
Tissue specificity
Expressed during myeloid lineage development. Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Highly expressed in pancreatic cancer tissues and shows an elevated expression level during the early S phase of the cell cycle. Also expressed at high levels in stomach cancer and colon cancer tissues.
Sequence similarities
Contains 1 olfactomedin-like domain.
Domain
The olfactomedin-like domain is involved in the interaction with cadherin.
Post-translational
modifications
N-glycosylated.
Cellular localization
Secreted > extracellular space. According to PubMed:15059901, found in mitochondrion and nucleus.
Target information above from: UniProt accessionQ6UX06
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Antiapoptotic protein GW112 antibody
- bA209J19.1 antibody
- G-CSF-stimulated clone 1 protein antibody
see all
Database links
- Entrez Gene: 515082 Cow
- Entrez Gene: 10562 Human
- SwissProt: B2RUF9 Cow
- SwissProt: Q6UX06 Human
- Unigene: 508113 Human
Anti-OLFM4 antibody images:
Western blot - OLFM4 antibody (ab65443)
Anti-OLFM4 antibody (ab65443) at 5 µg/ml + Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 57 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
References for Anti-OLFM4 antibody (ab65443)
ab65443 has not yet been referenced specifically in any publications.
Publishing research using ab65443? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"