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Western blot - OLFM4 antibody (ab65443)
Western blot - OLFM4 antibody (ab65443)
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Product Name 

OLFM4 antibody

 

See all OLFM4 antibodies (3)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to OLFM4

Immunogen 

A region within a synthetic peptide: AFMVCGVLYA TRTMNTRTEE IFYYYDTNTG KEGKLDIVMH KMQEKVQSIN, corresponding to C terminal amino acids 433-482 of Human OLFM4
BLAST 'AFMVCGVLYA TRTMNTRTEE IFYYYDTNTG KEGKLDIVMH KMQEKVQSIN ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay: 1/62500
WB: Use at a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 57 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Jurkat cell lysate.

Cellular localization 

Secreted, extracellular space

Research areas 

Cancer >> Tumor biomarkers >> Other

Relevance 

OLFM4 (olfactomedin 4) was originally cloned from human myeloblasts and found to be selectively expressed in inflammed colonic epithelium. OLFM4 (olfactomedin 4) is a member of the olfactomedin related protein family. The exact function of this gene has not yet been determined.

 

OLFM4 antibody (ab65443)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

10562    (Human)

SwissProt

    

Q6UX06    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this OLFM4 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for OLFM4 antibody images, references, products related to ab65443 and other tools.

 

OLFM4 antibody images:

Product image  Western blot - OLFM4 antibody (ab65443)

Western blot - OLFM4 antibody (ab65443)

OLFM4 antibody (ab65443) at 5 µg/ml + Jurkat cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 57 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)

 
 

Search PubMed (MEDLINE) for references to OLFM4

 

OLFM4 antibody - more information

Customer reviews (feedback) regarding OLFM4 antibody

Customer FAQs regarding OLFM4 antibody

Protocols for OLFM4 antibody

Price and availability of products related to OLFM4 antibody

 

OLFM4 antibody - related products:

Products for Western blot

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Lysates

 

Positive Controls for OLFM4 antibody

ab7899

  

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

 

Compatible Secondaries for OLFM4 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other OLFM4 Antibodies

See all OLFM4 antibodies (3)...

 

OLFM4 antibody - other tools:

Contact Abcam with a Technical Enquiry about OLFM4 antibody

 

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