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Products:Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Overview
Product name
Anti-OLIG1 antibody
See all Olig1 products (10) ...
Description
Mouse polyclonal to OLIG1
Tested applications
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
Fusion protein: MLRPQRPGDVQLGASLYELVGYRQPPISSSSSSSSSTASL LPKPAREKAEAPLAEPRGPAPESGGARADAKEEQQ, corresponding to amino acids 1/75 of Mouse OLIG1
MLRPQRPGDV QLGASLYELV GYRQPPISSS SSSSSSTASL LPKPAREKAE APLAEPRGPA PESGGARADA KEEQQ
General notes
Produced from outbred CD1 mice
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Constituents: 50% Glycerol
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
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Western blot - OLIG1 antibody (ab21943)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Predicted molecular weight: 30 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Target
Function
Promotes formation and maturation of oligodendrocytes, especially within the brain. Cooperates with OLIG2 to establish the pMN domain of the embryonic neural tube.
Tissue specificity
Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas is highly variable.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ8TAK6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Basic domain helix loop helix protein class B 6 antibody
- Basic domain helix loop helix protein class B6 antibody
- BHLH B6 antibody
see all
Database links
- Entrez Gene: 50914 Mouse
- Entrez Gene: 60394 Rat
- SwissProt: Q9JKN5 Mouse
- SwissProt: Q9WUQ3 Rat
- Unigene: 39300 Mouse
- Unigene: 45339 Rat
Anti-OLIG1 antibody images:
Western blot - OLIG1 antibody (ab21943)
All lanes : Anti-OLIG1 antibody (ab21943) at 1/1000 dilution
Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a
negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the
antigen fusion protein at 20 ug
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at
1/5000 dilution
Predicted band size : 30 kDa
The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.
References for Anti-OLIG1 antibody (ab21943)
ab21943 has not yet been referenced specifically in any publications.
Publishing research using ab21943? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"