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Western blot - OLIG1 antibody (ab21943)
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Product Name
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OLIG1 antibody
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See all Olig1 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to OLIG1
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Immunogen
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Fusion protein: MLRPQRPGDV QLGASLYELV GYRQPPISSS SSSSSSTASL LPKPAREKAE APLAEPRGPA PESGGARADA corresponding to amino acids 1/75 of Mouse OLIG1
BLAST 'MLRPQRPGDV QLGASLYELV GYRQPPISSS SSSSSSTASL LPKPAREKAE APLAEPRGPA PESGGARADA ' with  or 
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Reacts with
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Ms, Rat
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: 1/1000. Predicted molecular weight: 30 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
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Cellular localization
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Nuclear
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Research areas
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Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
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Relevance
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Olig1 is expressed in the brain, in oligodendrocytes. It is strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas is highly variable. Olig1 promotes formation and maturation of oligodendrocytes, especially within the brain. It cooperates with Olig2 to establish the pMN domain of the embryonic neural tube.
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein.
A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein.
Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Constituents: 50% Glycerol Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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Notes
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Produced from outbred CD1 mice
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this OLIG1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for OLIG1 antibody images, references, products related to ab21943 and other tools.
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OLIG1 antibody images:
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 Western blot - OLIG1 antibody (ab21943)
All lanes : OLIG1 antibody (ab21943) at 1/1000 dilution
Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a
negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the
antigen fusion protein at 20 ug
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at
1/5000 dilution
Predicted band size : 30 kDa
The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.
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Search PubMed (MEDLINE) for references to Olig1
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OLIG1 antibody - more information
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Customer reviews (feedback) regarding OLIG1 antibody |
Customer FAQs regarding OLIG1 antibody |
Protocols for OLIG1 antibody |
Price and availability of products related to OLIG1 antibody |
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OLIG1 antibody - related products:
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OLIG1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about OLIG1 antibody |
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