![Western blot - Anti-OPA1 antibody [1E81D9] (ab119685) image](#OPA1-Primary-antibodies-ab119685-2.jpg)
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Product name
Anti-OPA1 antibody [1E81D9]
See all OPA1 products (5) ...
Description
Mouse monoclonal [1E81D9] to OPA1
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Human, full-length, corresponding to short form of isoform 1
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% HBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. ab119685 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation.
Clonality
Monoclonal
Clone number
1E81D9
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Mitochondrial Metabolism >> Mitophagy fission and fusion
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Neuroscience >> Sensory System >> Visual system
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Western blot - Anti-OPA1 antibody [1E81D9] (ab119685)
![Western blot - Anti-OPA1 antibody [1E81D9] (ab119685) image](/ps/datasheet/images/119/ab119685/OPA1-Primary-antibodies-ab119685-2.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab119685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 112 kDa.
Target
Function
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Tissue specificity
Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues.
Involvement in disease
Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) [MIM:165500]. OPA1 is a dominantly inherited optic neuropathy occurring in 1 in 50,000 individuals that features progressive loss in visual acuity leading, in many cases, to legal blindness.
Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy.
Sequence similarities
Belongs to the dynamin family.
Post-translational
modifications
PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion.
Cellular localization
Mitochondrion inner membrane. Mitochondrion intermembrane space.
Target information above from: UniProt accessionO60313
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- form S1 antibody
- Dynamin like 120 kDa protein antibody
- Dynamin like 120 kDa protein, mitochondrial antibody
see all
Database links
- Entrez Gene: 4976 Human
- Entrez Gene: 74143 Mouse
- Entrez Gene: 171116 Rat
- Omim: 605290 Human
- SwissProt: O60313 Human
- SwissProt: P58281 Mouse
- SwissProt: Q2TA68 Rat
- Unigene: 594504 Human
see all
Anti-OPA1 antibody [1E81D9] images:
Western blot - Anti-OPA1 antibody [1E81D9] (ab119685)
All lanes : Anti-OPA1 antibody [1E81D9] (ab119685) at 1 µg/ml
Lane 1 : whole cell lysates from HeLa cells(human)
Lane 2 : whole cell lysates from H4IIE cells(rat)
Lane 3 : whole cell lysates from MEF cells(mouse)
Lysates/proteins at 30 µg per lane.
Secondary
HRP goat anti-mouse at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 112 kDa
References for Anti-OPA1 antibody [1E81D9] (ab119685)
ab119685 has not yet been referenced specifically in any publications.
Publishing research using ab119685? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"