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Product name
Anti-OS9 antibody
See all OS9 products (2) ...
Description
Rabbit polyclonal to OS9
Tested applications
WB, IP, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Dog
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human OS9.
(Peptide available as ab233 17.)
Positive control
This antibody gave a positive signal in the following Whole Cell lysates HeLa; HEK293; NIH3T3 and the following Mouse Tissue lysates Heart; Spleen: Liver. It also gave a positive signal in HeLa cell line in IF/ICC.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab19853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
IP: Use at an assay dependent dilution.
ICC/IF: Use a concentration of 5 µg/ml.
Target
Function
Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.
Tissue specificity
Ubiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma OSA-CL and rhabdomyosarcoma RH30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.
Sequence similarities
Belongs to the OS-9 family.
Contains 1 PRKCSH domain.
Post-translational
modifications
Intramolecular disulfide bonds.
Isoform 1 and isoform 2 are N-glycosylated.
Cellular localization
Endoplasmic reticulum lumen.
Target information above from: UniProt accessionQ13438
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Amplified in osteosarcoma 9 antibody
- amplified in osteosarcoma antibody
- OS-9 antibody
see all
Database links
- Entrez Gene: 10956 Human
- Entrez Gene: 216440 Mouse
- Entrez Gene: 362891 Rat
- Omim: 609677 Human
- SwissProt: Q13438 Human
- SwissProt: Q8K2C7 Mouse
- SwissProt: Q5RKH6 Rat
- Unigene: 527861 Human
see all
Anti-OS9 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - OS9 antibody (ab19853)
IHC image of OS9 staining in human hippocampus FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19853, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - OS9 antibody (ab19853)
All lanes : Anti-OS9 antibody (ab19853) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 4 : Spleen (Mouse) Tissue Lysate
Lane 5 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 76 kDa
Observed band size : 76 kDa
Additional bands at : 30 kDa,74 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 minutes
Immunocytochemistry/ Immunofluorescence - Anti-OS9 antibody (ab19853)
ICC/IF image of ab19853 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19853, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-OS9 antibody (ab19853)
This product has been referenced in:
- Christianson JC et al. OS-9 and GRP94 deliver mutant alpha1-antitrypsin to the Hrd1-SEL1L ubiquitin ligase complex for ERAD. Nat Cell Biol 10:272-82 (2008). WB, ICC/IF; Human.Read more (PubMed: 18264092) »
- Mueller B et al. SEL1L nucleates a protein complex required for dislocation of misfolded glycoproteins. Proc Natl Acad Sci U S A 105:12325-30 (2008). IP; Human.Read more (PubMed: 18711132) »
See all 2 publications for this product
Publishing research using ab19853? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"