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Anti-OTP antibody (ab50897)

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Overview

Product name

Anti-OTP antibody
See all OTP products (2) ...

Description

Rabbit polyclonal to OTP

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Cow, Dog, Zebrafish

Immunogen

A region within synthetic peptide: PAFPGMVPAS LPGPSNVSGS PQLCSSPDSS DVWRGTSIAS LRRKALEHTV, corresponding to amino acids 271-320 of Human OTP

PAFPGMVPAS LPGPSNVSGS PQLCSSPDSS DVWRGTSIAS LRRKALEHTV

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Jurkat cell lysate

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis

  • Western blot - OTP antibody (ab50897)Western blot - OTP antibody (ab50897) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab50897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay: 1/62500
WB: Use at a concentration of 0.5 µg/ml. Predicted molecular weight: 34 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

OTP is a member of the homeodomain (HD) family. HD family proteins are helix-turn-helix transcription factors that play key roles in the specification of cell fates. This protein may function during brain development.

Cellular localization

Nuclear

Alternative names

  • MGC3161 antibody
  • orthopedia homeobox antibody
  • orthopedia homolog antibody

Database links

Anti-OTP antibody images:

  Western blot - OTP antibody (ab50897)

Western blot - OTP antibody (ab50897)

Lane 1 : Marker
Lane 2 : Anti-OTP antibody (ab50897) at 0.5 µg/ml

Lane 1 : As above
Lane 2 : Jurkat cell lysate at 10 µg

Secondary
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


Predicted band size : 34 kDa


Gel concentration 12%

References for Anti-OTP antibody (ab50897)

ab50897 has not yet been referenced specifically in any publications.

Publishing research using ab50897? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"