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Form Lyophilised:Reconstitute in 200ul sterile water
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: 0.02% Sodium Azide
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 56 kDa.
1/100 - 1/500.
Function Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
Tissue specificity Abundant in heart, followed in order by kidney, brain, and muscle, whereas in liver it is undetectable; also detectable in leukocytes and fibroblasts.
Pathway Ketone metabolism; succinyl-CoA degradation; acetoacetyl-CoA from succinyl-CoA: step 1/1.
Involvement in disease Defects in OXCT1 are a cause of SCOT deficiency (SCOTD) [MIM:245050].
Sequence similarities Belongs to the 3-oxoacid CoA-transferase family.
Cellular localization Mitochondrion matrix.
Information by UniProt
3 oxoacid CoA transferase 1 antibody
3-oxoacid-CoA transferase 1 antibody
EC 188.8.131.52 antibody
Anti-OXCT1 antibody images
Western blot - Anti-OXCT1 antibody (ab105320) Image is courtesy of an AbReview submitted by Svitlana Yablonska.
All lanes : Anti-OXCT1 antibody (ab105320) at 1/1000 dilution Lane 1 : HEK293 whole cell lysate with SEA BLOCK blocking buffer Lane 2 : Mitochondria isolated from HEK293 cells with SEA BLOCK blocking buffer Lane 3 : Mitochondria isolated from non-synaptosomal human brain tissue. with SEA BLOCK blocking buffer Lane 4 : Mitochondria isolated from synaptosomal human neural tissue. with SEA BLOCK blocking buffer Lane 5 : Non-synaptosomal murine brain mitochondria (-ve control) with SEA BLOCK blocking buffer Lane 6 : Synaptosomal murine neural mitochondria (-ve control) with SEA BLOCK blocking buffer Lane 7 : STHdh-Q7/Q7 mitochondria (-ve control) with SEA BLOCK blocking buffer Lane 8 : STHdh-Q111/Q111 mitochondria (-ve control) with SEA BLOCK blocking buffer Lysates/proteins at 20 µg per lane. Blocking peptides at 100 % per lane. Secondary Goat anti-rabbit IRDye® 800CW at 1/30000 dilution Performed under reducing conditions. Predicted band size : 56 kDa Additional bands at : 20 kDa (possible non-specific binding),25 kDa (possible non-specific binding),52 kDa (possible post-translational modification),70 kDa (possible non-specific binding). Exposure time : 5 minutes Image is courtesy of an AbReview submitted by Svitlana Yablonska.
Western blot - OXCT1 antibody (ab105320)
All lanes : Anti-OXCT1 antibody (ab105320) at 1/500 dilution Lane 1 : Human fetal heart lysate Lane 2 : Human fetal brain lysate Predicted band size : 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - OXCT1 antibody (ab105320)
ab105320 at 1/100 dilution staining OXCT1 in Human fetal kidney tissue
References for Anti-OXCT1 antibody (ab105320)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"