Overview

  • Product nameAnti-OXCT1 antibody
    See all OXCT1 primary antibodies
  • Description
    Rabbit polyclonal to OXCT1
  • Tested applicationsWB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 171-434 of Human OXCT1 (BC009001).

  • Positive control
    • Human fetal heart and brain lysates; Human fetal kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab105320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 56 kDa.
ELISA 1/20000 - 1/80000.
IHC-P 1/100 - 1/500.

Target

  • FunctionKey enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
  • Tissue specificityAbundant in heart, followed in order by kidney, brain, and muscle, whereas in liver it is undetectable; also detectable in leukocytes and fibroblasts.
  • PathwayKetone metabolism; succinyl-CoA degradation; acetoacetyl-CoA from succinyl-CoA: step 1/1.
  • Involvement in diseaseDefects in OXCT1 are a cause of SCOT deficiency (SCOTD) [MIM:245050].
  • Sequence similaritiesBelongs to the 3-oxoacid CoA-transferase family.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 oxoacid CoA transferase 1 antibody
    • 3-oxoacid-CoA transferase 1 antibody
    • EC 2.8.3.5 antibody
    • mitochondrial antibody
    • OTTHUMP00000120012 antibody
    • OTTHUMP00000221550 antibody
    • OXCT antibody
    • Oxct1 antibody
    • SCOT antibody
    • Scot S antibody
    • Scot-S antibody
    • SCOT1_HUMAN antibody
    • Somatic type succinyl CoA:3 oxoacid CoA transferase antibody
    • Somatic-type succinyl-CoA:3-oxoacid-CoA-transferase antibody
    • Succinyl CoA:3 ketoacid CoA transferase antibody
    • Succinyl CoA:3 ketoacid coenzyme A transferase 1 mitochondrial antibody
    • Succinyl CoA:3 oxoacid CoA transferase antibody
    • Succinyl-CoA:3-ketoacid-coenzyme A transferase 1 antibody
    • Succinyl-CoA:3-ketoacid-coenzyme A transferase 1, mitochondrial antibody
    see all

Anti-OXCT1 antibody images

  • All lanes : Anti-OXCT1 antibody (ab105320) at 1/500 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal brain lysate


    Predicted band size : 56 kDa
  • ab105320 at 1/100 dilution staining OXCT1 in Human fetal kidney tissue

References for Anti-OXCT1 antibody (ab105320)

ab105320 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - other (Mice brain, and cell line mitochondria)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 20 µg
Specification Mice brain, and cell line mitochondria
Blocking step SEA BLOCK Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Svitlana Yablonska

Verified customer

Submitted Aug 24 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"