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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-OXCT1 antibody
See all OXCT1 products (3) ...
Description
Mouse polyclonal to OXCT1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length OXCT1 protein (NP_000427.1) (Human)
Positive control
OXCT1 transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - OXCT1 antibody (ab70413)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 56 kDa.
Target
Function
Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
Tissue specificity
Abundant in heart, followed in order by kidney, brain, and muscle, whereas in liver it is undetectable; also detectable in leukocytes and fibroblasts.
Pathway
Ketone metabolism; succinyl-CoA degradation; acetoacetyl-CoA from succinyl-CoA: step 1/1.
Involvement in disease
Defects in OXCT1 are a cause of SCOT deficiency (SCOTD) [MIM:245050].
Sequence similarities
Belongs to the 3-oxoacid CoA-transferase family.
Cellular localization
Mitochondrion matrix.
Target information above from: UniProt accessionP55809
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 3 oxoacid CoA transferase 1 antibody
- 3-oxoacid-CoA transferase 1 antibody
- EC 2.8.3.5 antibody
see all
Database links
- Entrez Gene: 5019 Human
- Omim: 601424 Human
- SwissProt: P55809 Human
- Unigene: 278277 Human
Anti-OXCT1 antibody images:
Western blot - OXCT1 antibody (ab70413)
All lanes : Anti-OXCT1 antibody (ab70413) at 1/500 dilution
Lane 1 : OXCT1 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 56 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
References for Anti-OXCT1 antibody (ab70413)
ab70413 has not yet been referenced specifically in any publications.
Publishing research using ab70413? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"