Anti-Occludin antibody (ab222691)
Key features and details
- Rabbit polyclonal to Occludin
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Human
- Isotype: IgG
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Overview
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Product name
Anti-Occludin antibody
See all Occludin primary antibodies -
Description
Rabbit polyclonal to Occludin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Pig -
Immunogen
Synthetic peptide within Mouse Occludin aa 480-520 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q61146 -
Positive control
- WB: A549 cell lysate and Mouse kidney lysates. Flow Cyt: 293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/100.
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WB |
1/100 - 1/1000. Predicted molecular weight: 59 kDa.
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Notes |
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Flow Cyt
1/100. |
WB
1/100 - 1/1000. Predicted molecular weight: 59 kDa. |
Target
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Function
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. -
Tissue specificity
Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. -
Involvement in disease
Defects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:251290]; also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay. -
Sequence similarities
Belongs to the ELL/occludin family.
Contains 1 MARVEL domain. -
Domain
The C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. -
Cellular localization
Membrane. Cell junction > tight junction. - Information by UniProt
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Database links
- Entrez Gene: 100506658 Human
- Entrez Gene: 18260 Mouse
- Entrez Gene: 397236 Pig
- Entrez Gene: 83497 Rat
- Omim: 602876 Human
- SwissProt: Q16625 Human
- SwissProt: Q61146 Mouse
- SwissProt: Q6P6T5 Rat
see all -
Alternative names
- BLCPMG antibody
- FLJ08163 antibody
- FLJ18079 antibody
see all
Images
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Anti-Occludin antibody (ab222691) at 1/300 dilution + Mouse kidney tissue lysate
Secondary
A conjugated secondary antibody at 1/5000 dilution
Predicted band size: 59 kDa -
Flow Cytometry analysis of 293T cells labelling Occludin with ab222691 at 1/100 for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
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Anti-Occludin antibody (ab222691) at 1/300 dilution + A549 cell lysate
Secondary
Goat Anti-Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 59 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab222691 has been referenced in 15 publications.
- Cui YJ et al. Heat stress induced IPEC-J2 cells barrier dysfunction through endoplasmic reticulum stress mediated apoptosis by p-eif2α/CHOP pathway. J Cell Physiol 237:1389-1405 (2022). PubMed: 34661912
- Wang G et al. Icariin alleviates uveitis by targeting peroxiredoxin 3 to modulate retinal microglia M1/M2 phenotypic polarization. Redox Biol 52:102297 (2022). PubMed: 35334248
- Kimura S et al. Histological characteristics of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinases-1 in asthmatic murine model during A(H1N1)pdm09 infection. Pathol Int 72:506-518 (2022). PubMed: 36066006
- Wang Y et al. Sini Decoction Ameliorates Colorectal Cancer and Modulates the Composition of Gut Microbiota in Mice. Front Pharmacol 12:609992 (2021). PubMed: 33776762
- Wang W et al. NEO100 enables brain delivery of blood?brain barrier impermeable therapeutics. Neuro Oncol 23:63-75 (2021). PubMed: 32877532