Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> OCT
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ab20650 |
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ab27985 has been referenced in 9 publications.
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All lanes : Anti-Oct4 antibody - Embryonic Stem Cell Marker (ab27985) at 1 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate
Lane 2 : Mouse Embryonic Stem Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit polyclonal to Goat IgG (Alexa Fluor® 680) (ab28445) at 1/10000 dilution
Predicted band size : 39 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
The antibody recognised a band corresponding to Oct4 in mouse and human embryonic stem cell lysates.
ab27985 recognizes Oct4 in human embryonic stem cells. The upper panel shows DAPI staining while the lower panel shows ab27985 staining.
This image is courtesy of an Abreview submitted by Dr Maria Keramari
ab27985 recognizes Oct4 in mouse embryonic stem cells. DAPI staining is shown in the top panel while ab27985 staining is shown in the lower panel.
This image is courtesy of an Abreview submitted by Dr Maria Keramari
ab27985 staining Oct4 in human embryonic carcinoma cells by Flow Cytometery. Cells were fixed in paraformaldehyde and permeabilized in saponin buffer. The primary antibody was diluted 1/500 (PBS + 10% BSA) and incubated with sample for 1 hour at 4°C. A FITC conjugated Donkey polyclonal to goat IgG, diluted 1/200 was used as secondary. The red histogram is secondary only control whilst green and blue repersents Oct4 expression in untreated and partially differentiated with 10nM Trans-Retinoic acid for 14 days.
This image is courtesy of an Abreview submitted by Mr Mark Jones
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