Anti-Oct4 antibody [GT486] (ab184665)
Key features and details
- Mouse monoclonal [GT486] to Oct4
- Suitable for: ICC/IF, Flow Cyt, IP, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Oct4 antibody [GT486]
See all Oct4 primary antibodies -
Description
Mouse monoclonal [GT486] to Oct4 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee -
Immunogen
Recombinant fragment within Human Oct4 aa 1-182. The exact sequence is proprietary.
Database link: Q01860 -
Positive control
- Oct4 transfected 293T and HeLa cells; NT2D1 whole cell lysates; mouse and Human ESC whole cell lysates; Human embryonic stem cells and BT483 xenograft tissue.
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General notes
This product was changed from ascites to tissue culture supernatant on 17th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Constituents: 80% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
GT486 -
Isotype
IgG2b -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (1) |
1/100 - 1/1000.
|
Flow Cyt |
1/50.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
IP |
1/100 - 1/500.
|
|
WB |
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
|
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IHC-P |
1/100 - 1/1000.
|
Notes |
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ICC/IF
1/100 - 1/1000. |
Flow Cyt
1/50. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
IP
1/100 - 1/500. |
WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. -
Tissue specificity
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. -
Sequence similarities
Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Developmental stage
Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation. -
Domain
The POU-specific domain mediates interaction with PKM2. -
Post-translational
modificationsSumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. -
Cellular localization
Nucleus. Expressed in a diffuse and slightly punctuate pattern. - Information by UniProt
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Database links
- Entrez Gene: 744263 Chimpanzee
- Entrez Gene: 5460 Human
- Entrez Gene: 18999 Mouse
- Omim: 164177 Human
- SwissProt: Q7YR49 Chimpanzee
- SwissProt: Q01860 Human
- SwissProt: P20263 Mouse
- Unigene: 249184 Human
see all -
Alternative names
- Octamer binding transcription factor 4 antibody
- MGC22487 antibody
- Oct 3 antibody
see all
Images
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Immunofluorescence analysis of 4% paraformaldehyde-fixed Human embryonic stem cells labeling Oct4 with ab184665 at 1/200 dilution (green). Hoechst 33342 staining is shown in blue.
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Immunoprecipitation of Oct4 transfected 293T cell lysate followed by detection of Oct4 with ab184665 at 1/1000 dilution.
Lane 1; Cell lysate/extract of transfected 293T cells.Lane 2; Cell lysate/extract of transfected 293T cells precipitated with 2 μg of preimmune mouse IgG (control)
Lane 3; Cell lysate/extract of transfected 293T cells precipitated using 2 µg of ab184665.
Detection utilised anti-mouse IgG. Samples analysed by 12% SDS-PAGE. -
Anti-Oct4 antibody [GT486] (ab184665) at 1/1000 dilution + Mouse ESC whole cell lysate at 20 µg
Predicted band size: 39 kDa10% SDS PAGE
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Anti-Oct4 antibody [GT486] (ab184665) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg
Predicted band size: 39 kDa10% SDS PAGE
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All lanes : Anti-Oct4 antibody [GT486] (ab184665) at 1/1000 dilution
Lane 1 : Non-transfected HeLa whole cell lysates
Lane 2 : OCT3/4 transfected HeLa whole cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa10% SDS PAGE
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Anti-Oct4 antibody [GT486] (ab184665) at 1/1000 dilution + Human ESC whole cell lysate/extract at 30 µg
Predicted band size: 39 kDa10 % SDS-PAGE
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Flow cytometric analysis of Human embryonic stem cells labeling Oct4 with ab184665 at 1/50 dilution (green). The isotype control was at 1/50 dilution (Black).
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Immunohistochemical analysis of paraffin-embedded BT483 (human) xenograft tissue labeling Oct4 with ab184665 at 1/200 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab184665 has been referenced in 23 publications.
- Zhang W et al. Retinoic Acid-Induced 2 (RAI2) Is a Novel Antagonist of Wnt/β-Catenin Signaling Pathway and Potential Biomarker of Chemosensitivity in Colorectal Cancer. Front Oncol 12:805290 (2022). PubMed: 35299743
- Fagg WS et al. Definition of germ layer cell lineage alternative splicing programs reveals a critical role for Quaking in specifying cardiac cell fate. Nucleic Acids Res 50:5313-5334 (2022). PubMed: 35544276
- Zhang Q et al. Folic Acid Preconditioning Alleviated Radiation-Induced Ovarian Dysfunction in Female Mice. Front Nutr 9:854655 (2022). PubMed: 35836584
- Raina K et al. Stage-Specific Effect of Inositol Hexaphosphate on Cancer Stem Cell Pool during Growth and Progression of Prostate Tumorigenesis in TRAMP Model. Cancers (Basel) 14:N/A (2022). PubMed: 36077751
- Ma Z et al. Activation of embryonic/germ cell-like axis links poor outcomes of gliomas. Cancer Cell Int 22:371 (2022). PubMed: 36435765