Anti-Oct4 antibody (ab137427)
Key features and details
- Rabbit polyclonal to Oct4
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Oct4 antibody
See all Oct4 primary antibodies -
Description
Rabbit polyclonal to Oct4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Cow, Cat, Pig, Monkey -
Immunogen
Recombinant fragment corresponding to Human Oct4 aa 1-182.
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Positive control
- Recombinant human Oct4 protein (ab134876) can be used as a positive control in WB. HeLa, Human embryonic stem cells (ESC) and Mouse ESC whole cell lysates; HeLa nuclear cell lysate; Human embryonic stem cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Affinity Purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
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Flow Cyt |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa. |
Flow Cyt
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. -
Tissue specificity
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. -
Sequence similarities
Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Developmental stage
Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation. -
Domain
The POU-specific domain mediates interaction with PKM2. -
Post-translational
modificationsSumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. -
Cellular localization
Nucleus. Expressed in a diffuse and slightly punctuate pattern. - Information by UniProt
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Database links
- Entrez Gene: 282316 Cow
- Entrez Gene: 5460 Human
- Entrez Gene: 18999 Mouse
- Entrez Gene: 100127461 Pig
- Entrez Gene: 100101567 Rabbit
- Omim: 164177 Human
- SwissProt: O97552 Cow
- SwissProt: Q01860 Human
see all -
Alternative names
- Octamer binding transcription factor 4 antibody
- MGC22487 antibody
- Oct 3 antibody
see all
Images
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Anti-Oct4 antibody (ab137427) at 1/5000 dilution + Mouse Embryonic Stem Cell whole cell lysate
at 20 µg
Predicted band size: 38 kDa
12% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed Human Embryonic Stem cells labelling Oct4 with ab137427 at 1:400 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunofluorescent analysis of paraformaldehyde-fixed Human embryonic stem cells labelling Oct4 with ab137427 at 1:200 dilution.
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Immunofluorescent analysis of paraformaldehyde-fixed Human embryonic stem cells labelling Oct4 with ab137427 at 1:100 dilution. Lower image shows cells co-stained with DAPI.
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Anti-Oct4 antibody (ab137427) at 1/1000 dilution + HeLa nuclear cell lysate at 20 µg
Predicted band size: 38 kDa
10% SDS PAGE -
Anti-Oct4 antibody (ab137427) at 1/1000 dilution + Human Embryonic Stem Cell whole cell lysate at 20 µg
Predicted band size: 38 kDa
10% SDS PAGE -
Flow cytometric analysis of Human Embryonic Stem cells labelling Oct4 with ab137427 at 1:50 dilution (green) or rabbit IgG (black).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab137427 has been referenced in 14 publications.
- Arcuri S et al. Generation of Trophoblast-Like Cells From Hypomethylated Porcine Adult Dermal Fibroblasts. Front Vet Sci 8:706106 (2021). PubMed: 34350230
- Zhao X et al. TNF-a promotes the malignant transformation of intestinal stem cells through the NF-?B and Wnt/ß-catenin signaling pathways. Oncol Rep 44:577-588 (2020). PubMed: 32627006
- Flamini MA et al. Key characteristics of the ovary and uterus for reproduction with particular reference to poly ovulation in the plains viscacha (Lagostomus maximus, chinchillidae). Theriogenology 142:184-195 (2020). PubMed: 31604179
- Dong W et al. SATB2 knockdown decreases hypoxia-induced autophagy and stemness in oral squamous cell carcinoma. Oncol Lett 20:794-802 (2020). PubMed: 32566006
- Lima DBC et al. Long-term Preservation of Testicular Tissue Integrity and Viability Using Vitrification in the Endangered Black-Footed Ferret (Mustela Nigripes). Animals (Basel) 10:N/A (2020). PubMed: 33066219