Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> OCT
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ab52110 |
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ab18976 has been referenced in 11 publications.
Publishing research using ab18976? Please let us know so that we can cite the reference in this datasheet
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Anti-Oct4 antibody (ab18976) at 1/500 dilution + mouse embryonic stem cell whole cell lysate at 100 µg/ml
Secondary
HRP conjugated goat anti-rabbit
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 45 kDa
Observed band size : 45 kDa
Additional bands at : 25 kDa (possible non-specific binding),60 kDa (possible non-specific binding).
Exposure time : 1 minute
The blot was blocked with 5% milk for 1 hour at RT and inbubated with the primary antibody for 16 hours at 4°C.
This image is courtesy of an anonymous Abreview
Anti-Oct4 antibody (ab18976) at 0.5 µg/ml + Lysates prepared from rat kidney tissue.
Predicted band size : 45 kDa
ab18976 at 1/100 dilution staining Oct4 in mouse brain tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed prior to blocking in 5% BSA for 1 hour at 25ºC and then incubated with ab18976 for 12 hours at 4ºC. A Cy3 conjugated donkey polyclonal to rabbit Ig, diluted 1/500, was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
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