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Western blot - Oct4 antibody (ab18976)
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Western blot - Oct4 antibody (ab18976)
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Product Name
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Oct4 antibody
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See all Oct4 antibodies (15)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Oct4
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Immunogen
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Recombinant fragment, corresponding to amino acids 1-140 of Human Oct4(Peptide available as ab52110.)
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Reacts with
(species key)
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Hu, Ms, Rat, Cow, Pig
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution (PMID 19736565).
IHC-P: 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Use at a concentration of 20 µg/ml.
IP: Use at a concentration of 20 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 45 kDa. Can be blocked with Oct4 peptide (ab52110).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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F9 whole cell lysate
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Stem Cells >> Embryonic Stem Cells >> Intracellular
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> OCT
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
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Relevance
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Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation. Oct4 is renowned as a transcription factor expressed by undifferentiated embryonic stem cells and embryonic germ cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.2 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Oct4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Oct4 antibody images, references, products related to ab18976 and other tools.
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Oct4 antibody images:
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 Western blot - Oct4 antibody (ab18976)
Oct4 antibody (ab18976) at 1/500 dilution + mouse embryonic stem cell whole cell lysate at 100 µg/ml
Secondary
HRP conjugated goat anti-rabbit
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 45 kDa
Observed band size : 45 kDa
Additional bands at : 25 kDa (possible non-specific binding),60 kDa (possible non-specific binding).
Exposure time : 1 minute
The blot was blocked with 5% milk for 1 hour at RT and inbubated with the primary antibody for 16 hours at 4°C. This image is courtesy of an anonymous Abreview
See Abreview
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Western blot - Oct4 antibody (ab18976)
Oct4 antibody (ab18976) at 0.5 µg/ml + Lysates prepared from rat kidney tissue.
Predicted band size : 45 kDa
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References for Oct4 antibody (ab18976)
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This product has been used in: (two most recent references)
Vallier L et al. Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways. PLoS One 4:e6082 (2009). ICC/IF; Human. PubMed: 19564924
Song Z et al. Efficient generation of hepatocyte-like cells from human induced pluripotent stem cells. Cell Res 19:1233-42 (2009). ICC/IF; Human. PubMed: 19736565
See all 3 publication references for this product.
If you publish research using ab18976 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Oct4
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Oct4 antibody - more information
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Customer reviews (feedback) regarding Oct4 antibody |
Customer FAQs regarding Oct4 antibody |
Protocols for Oct4 antibody |
Price and availability of products related to Oct4 antibody |
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Oct4 antibody - related products:
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Oct4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Oct4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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