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Overview

  • Product nameAnti-Oct4 antibodySee all Oct4 primary antibodies ...
  • Description
    Rabbit polyclonal to Oct4
  • SpecificityDetects endogenous levels of total Oct4 protein.
  • Tested applicationsSandwich ELISA, WB, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Human)

  • Positive controlHeLa cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab53028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA sELISA: Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal to Oct4 (ab77259). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal to Oct4 (ab77259) as Capture.
WB WB: 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 39 kDa).
ELISA ELISA: 1/10000.

Target

  • FunctionTranscription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • Tissue specificityExpressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
  • Sequence similaritiesBelongs to the POU transcription factor family. Class-5 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Developmental stageHighly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
  • DomainThe POU-specific domain mediates interaction with PKM2.
  • Post-translational
    modifications    Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
  • Cellular localizationNucleus. Expressed in a diffuse and slightly punctuate pattern.

    • Target information above from: UniProt accession Q01860 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      MGC22487 antibodyOct 3 antibodyOct 4 antibody
      Oct-3 antibodyOct-4 antibodyOCT3 antibodyOct4 antibodyOctamer binding protein 3 antibodyOctamer binding protein 4 antibodyOctamer binding transcription factor 3 antibodyOctamer-binding protein 3 antibodyOctamer-binding protein 4 antibodyOctamer-binding transcription factor 3 antibodyOTF 3 antibodyOTF 4 antibodyOTF-3 antibodyOTF3 antibodyOTF4 antibodyPO5F1_HUMAN antibodyPOU class 5 homeobox 1 antibodyPOU domain class 5 transcription factor 1 antibodyPOU domain transcription factor OCT4 antibodyPOU domain, class 5, transcription factor 1 antibodyPOU type homeodomain containing DNA binding protein antibodyPOU5F1 antibody
    see all

Anti-Oct4 antibody images

  • Sandwich ELISA - Oct4 antibody (ab53028)
    Sandwich ELISA - Oct4 antibody (ab53028)
    Standard Curve for Oct4; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal to Oct4 (ab77259) at 5 ug/ml and Detector Antibody Rabbit polyclonal to Oct4 (ab53028) at 0.5 ug/ml.
  • Western blot - Oct4 antibody (ab53028)
    Western blot - Oct4 antibody (ab53028)
    All lanes : Anti-Oct4 antibody (ab53028) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with immunising peptide


    Predicted band size : 39 kDa

Protocols

References for Anti-Oct4 antibody (ab53028)

ab53028 has not yet been referenced specifically in any publications.

Publishing research using ab53028 ? Please let us know so that we can cite the reference in this datasheet

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Customer kindly contacted us desiring an unconjugated sandwich ELISA antibody pair that detects a protein involved in cell differentiation and proliferation, as well as a fluorescent secondary. Ideally, the protein would be available for purchase as well.

Thank you for contacting Abcam.

I have identified a pair of antibodies that have been tested and guaranteed to work in sandwich ELISA against the human Oct4 protein, a protein involved in stem cell self-renewal. Links to the antibodies are given ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"