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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> OCT
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Related products
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Primary antibodies
ab19857
ab19857
ab19857
ab19857
ab19857
ab19857
ab19857
ab19857
ab19857
ab19857
ab27985
Goat polyclonal to Oct4 - Embryonic Stem Cell Marker (ab27985)
ab80700
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab20650? Please let us know so that we can cite the reference in this datasheet
ab20650 has been referenced in 1 publications.
- Salmina K et al. Up-regulation of the embryonic self-renewal network through reversible polyploidy in irradiated p53-mutant tumour cells. Exp Cell Res 316:2099-112 (2010). Blocking. PubMed: 20457152
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot
All lanes : Anti-Oct4 antibody - ChIP Grade (ab19857) at 1 µg/ml
Lane 1 : Human embryonic stem cell lysate
Lane 2 : Mouse embryonic stem cell lysate
Lane 3 : Mouse embryonic germ cell lysate
Lane 4 : Human embryonic stem cell lysate with
Lane 5 : Mouse embryonic stem cell lysate with
Lane 6 : Mouse embryonic germ cell lysate with
Lysates/proteins at 20 µg per lane.
Predicted band size : 39 kDa
Observed band size : 43 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa (possible glycosylated form,cross reactivity).
Anti-Oct4 antibody ab19857 only detected a band corresponding to the expected size of Oct4 in human ES cell lysate. In mouse ES and EG cell lysates, ab19857 detected a band of approximately 55 kDa in addition to the expected 39 kDa Oct4 band.
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