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Overview

  • Product nameOct4 peptideSee all Oct4 proteins and peptides ...
  • Protein descriptionSynthetic peptide derived from within residues 300 to the C-terminus of Human Oct4.(Note: the amino acid sequence is proprietary)This peptide was used as an immunogen for ab19857 - Oct4 antibody - ChIP Grade, ab27985 - Oct4 antibody - Embryonic Stem Cell Marker and ab80700 - Oct4 antibody.
  • Protein length360 amino acids

    • Properties

  • Purity70 - 90% by HPLC
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferInformation available upon request.
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab20650 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Blocking
  • Application notesBL: Use at a concentration of 1 µg/ml.
    This peptide can be used with studies using ab19857.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        MGC22487Oct 3Oct 4
        Oct-3Oct-4OCT3Oct4Octamer binding protein 3Octamer binding protein 4Octamer binding transcription factor 3Octamer-binding protein 3Octamer-binding protein 4Octamer-binding transcription factor 3OTF 3OTF 4OTF-3OTF3OTF4PO5F1_HUMANPOU class 5 homeobox 1POU domain class 5 transcription factor 1POU domain transcription factor OCT4POU domain, class 5, transcription factor 1POU type homeodomain containing DNA binding proteinPOU5F1
      see all
  • FunctionTranscription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • Tissue specificityExpressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
  • Sequence similaritiesBelongs to the POU transcription factor family. Class-5 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Developmental stageHighly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
  • DomainThe POU-specific domain mediates interaction with PKM2.
  • Post-translational
    modifications    Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
  • Cellular localizationNucleus. Expressed in a diffuse and slightly punctuate pattern.

    • Target information above from: UniProt accession Q01860 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Oct4 peptide images

    • Western blot
      Western blot
      All lanes : Anti-Oct4 antibody - ChIP Grade (ab19857) at 1 µg/ml

      Lane 1 : Human embryonic stem cell lysate
      Lane 2 : Mouse embryonic stem cell lysate
      Lane 3 : Mouse embryonic germ cell lysate
      Lane 4 : Human embryonic stem cell lysate with Oct4 peptide (ab20650) at 1 µg/ml
      Lane 5 : Mouse embryonic stem cell lysate with Oct4 peptide (ab20650) at 1 µg/ml
      Lane 6 : Mouse embryonic germ cell lysate with Oct4 peptide (ab20650) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.


      Predicted band size : 39 kDa
      Observed band size : 43 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 55 kDa (possible glycosylated form,cross reactivity).      Anti-Oct4 antibody ab19857 only detected a band corresponding to the expected size of Oct4 in human ES cell lysate. In mouse ES and EG cell lysates, ab19857 detected a band of approximately 55 kDa in addition to the expected 39 kDa Oct4 band.

    References for Oct4 peptide (ab20650)

    This product has been referenced in:
    • Salmina K  et al. Up-regulation of the embryonic self-renewal network through reversible polyploidy in irradiated p53-mutant tumour cells. Exp Cell Res 316:2099-112 (2010). Blocking . Read more (PubMed: 20457152) »

    See 1 Publication for this product

    Publishing research using ab20650 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

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    I have an enquiry regarding your products; ab20650 and ab19857.
    I would like to confirm with you that ab20650 can be used to elute
    Oct4 proteins immunoprecipitated by ab19857.
    I would be most grateful if you could allow me yo know
    whet...

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    Thank you for contacting us.

    The peptide ab20650 has not been specifically validated for use eluting proteins that were immunoprecipitated using ab19857, but it should be suitable for this purpose when used at a high enough concentration. I woul...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"