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Oct4 peptide (ab20650)

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Overview

Product name

Oct4 peptide
See all Oct4 products (3) ...

Protein description

Synthetic peptide derived from within residues 300 to the C-terminus of Human Oct4.(Note: the amino acid sequence is proprietary)This peptide was used as an immunogen for ab19857 - Oct4 antibody - ChIP Grade, ab27985 - Oct4 antibody - Embryonic Stem Cell Marker and ab80700 - Oct4 antibody.

Protein length

360 amino acids

Properties

Purity

70 - 90% by HPLC

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Information available upon request.

Concentration

Concentration information loading...

Research areas

Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Stem Cells >> Embryonic Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> OCT

Applications

Show applications key

Our Abpromise guarantee covers the use of ab20650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Blocking

     

Application notes

BL: Use at a concentration of 1 µg/ml.
This peptide can be used with studies using ab19857.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Protein info

Function

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Tissue specificity

Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

Sequence similarities

Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.

Developmental stage

Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.

Domain

The POU-specific domain mediates interaction with PKM2.

Post-translational
modifications

Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.

Cellular localization

Nucleus. Expressed in a diffuse and slightly punctuate pattern.

Target information above from: UniProt accessionQ01860 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Oct4 peptide images:

  Western blot

Western blot

All lanes : Anti-Oct4 antibody - ChIP Grade (ab19857) at 1 µg/ml

Lane 1 : Human embryonic stem cell lysate
Lane 2 : Mouse embryonic stem cell lysate
Lane 3 : Mouse embryonic germ cell lysate
Lane 4 : Human embryonic stem cell lysate with Oct4 peptide (ab20650) at 1 µg/ml
Lane 5 : Mouse embryonic stem cell lysate with Oct4 peptide (ab20650) at 1 µg/ml
Lane 6 : Mouse embryonic germ cell lysate with Oct4 peptide (ab20650) at 1 µg/ml

Lysates/proteins at 20 µg per lane.


Predicted band size : 39 kDa
Observed band size : 43 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa (possible glycosylated form,cross reactivity).

Anti-Oct4 antibody ab19857 only detected a band corresponding to the expected size of Oct4 in human ES cell lysate. In mouse ES and EG cell lysates, ab19857 detected a band of approximately 55 kDa in addition to the expected 39 kDa Oct4 band.

References for Oct4 peptide (ab20650)

This product has been referenced in:

  • Salmina Ket al. Up-regulation of the embryonic self-renewal network through reversible polyploidy in irradiated p53-mutant tumour cells. Exp Cell Res 316:2099-112 (2010). Blocking.Read more (PubMed: 20457152) »

See 1 publication for this product

Publishing research using ab20650? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"