Anti-Octopamine (conjugated ) antibody (ab37092)
Overview
- Product nameAnti-Octopamine (conjugated ) antibody
- DescriptionRabbit polyclonal to Octopamine (conjugated )
- SpecificityThis antibody targets conjugated octopamine. This antibody does not recognize free octopamine. Using a conjugate octopamine-protein carrier (Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds: cross reactivity§ Octopamine-G-(Pc):1 Tyramine-G-(Pc):1/400 Naradrenaline-G-(Pc):1/10,000 Tyrosine-G-(Pc):1/>50,000 Dopamine-G-(Pc):1/100,000 G = Glutaraldehyde § Octopamine-G-(Pc) concentration/other conjugated catecholamine concentration at half displacement.
- Tested applicationsIHC-Glut, ELISA, ICC, WB more details
- Species reactivitynot applicable
- Immunogen
Synthetic octopamine conjugated to protein carrier
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 50% Glycerol, ddH20 -
Concentration information loading... - Purification notesAntiserum previously preabsorbed on protein carriers and purified.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab37092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-Glut | IHC-Glut: 1/1000 - 1/5000. Fixation should be done with gluatraldehyde. The use of PFA with glutaraldehyde may improve staining results. |
| ELISA | ELISA: 1/1000 - 1/5000. |
| ICC | ICC: 1/1000 - 1/5000. Fixation should be done with gluatraldehyde. The use of PFA with glutaraldehyde may improve staining results. |
| WB | WB: 1/1000 - 1/2000. Predicted molecular weight: 1 kDa. This antibody does not recognize free octopamine. |
Target
- RelevanceOctopamine was first discovered in the salivary glands of the octopus and has since been found to act as neurotransmitter, neurohormone and neuromodulator in invertebrates. Octopamine is a naturally occouring selective beta-3 adrenergic recepter agonist, which has been shown to exhibit strong lipolytic activity in fat cells. Octopamine also replaces norepinephrine in sympathetic neurons with chronic use of Monoamine Oxidase Inhibitors. It is responsible for the common side-effect profile of orthostatic hypotension with these agents.
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Alternative names
- 1 (4 hydroxyphenyl) 2 aminoethanol antibodyalpha(aminomethyl) 4 hydroxy benzenemethanol antibodyOctopamine antibody
References for Anti-Octopamine (conjugated ) antibody (ab37092)
This product has been referenced in:
- Châtel A et al. Characterization of tyramine ß-hydroxylase, an enzyme upregulated by stress in Periplaneta americana. J Mol Endocrinol 50:91-102 (2013). ELISA . Read more (PubMed: 23197360) »
