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Immunocytochemistry/ Immunofluorescence - Ogg1 antibody (ab204)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ogg1 antibody (ab204)
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Product Name
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Ogg1 antibody
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See all Ogg1 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Ogg1
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Immunogen
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Synthetic peptide: CTVYRGDKSQASRPT conjugated to KLH, corresponding to amino acids 75-89 of Human Ogg1.
BLAST 'CTVYRGDKSQASRPT' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat, Cow, Dog
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Specificity
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This antibody is specific for hOgg1.
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Epitope
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CTVYRGDKSQASRPT Human
CTVYRGDKGQVGRPT Rat
CTVYRGDDSQVSRPT Mouse
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Tested applications
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ICC/IF, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/50 (see Abreview for further information).
IHC-P: 1/25 - 1/100.
IHC-Fr: 1/25 - 1/100.
WB: 1/500 - 1/1000. Predicted molecular weight: 39 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
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WB - Jurkat whole cell extracts.
IHC - Human tonsil.
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Cellular localization
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Mitochondrial and Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
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Relevance
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The Ogg1 gene encodes a DNA repair enzyme (N glycosylase/DNA lyase) that incises DNA at 8 oxoG residues. N glycosylase/DNA lyase excises 7,8 dihydro 8 oxoguanine and 2,6 diamino 4 hydroxy 5 N methylformamidopyrimidine (FAPY) from damaged DNA. N glycosylase/DNA lyase has a beta lyase activity that nicks DNA 3' to the lesion.
Alternative splicing of the C terminal region of the Ogg1 gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N terminal region in common. Many alternative splice variants for this gene have been described, but the full length nature for every variant has not been determined. The N terminus of this gene contains a mitochondrial targetting signal, essential for mitochondrial localization.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS with 0.05% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.100 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Ogg1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Ogg1 antibody images, references, products related to ab204 and other tools.
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Ogg1 antibody images:
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 Immunocytochemistry/ Immunofluorescence - Ogg1 antibody (ab204)
ab204 at a 1/50 dilution staining primary human choroidal endothelial cells, stimulated to express Ogg1 using H 2O2. The antibody was incubated with the cells for 3 hours and then detected using an Alexa-Fluor ® 488 goat anti-rabbit (IgG). This image is courtesy of an Abreview submitted on 1 March 2006.
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ogg1 antibody (ab204)
Ab204 staining Human normal colon. Staining is localized to the nucleus.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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References for Ogg1 antibody (ab204)
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This product has been used in: (two most recent references)
Chatterjee A et al. The effect of p53-RNAi and p53 knockout on human 8-oxoguanine DNA glycosylase (hOgg1) activity. FASEB J 20:112-4 (2006). PubMed: 16293709
Mambo E et al. Oxidized guanine lesions and hOgg1 activity in lung cancer. Oncogene 24:4496-508 (2005). PubMed: 15856018
If you publish research using ab204 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Ogg1
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Ogg1 antibody - more information
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Customer reviews (feedback) regarding Ogg1 antibody |
Customer FAQs regarding Ogg1 antibody |
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Price and availability of products related to Ogg1 antibody |
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Ogg1 antibody - related products:
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Ogg1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Ogg1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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