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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Overview
Product name
Anti-Ogg1 antibody
See all Ogg1 products (7) ...
Description
Rabbit polyclonal to Ogg1
Tested applications
WB, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Ogg1.
(Peptide available as ab23381.)
Positive control
This antibody gave a positive signal in the following lysates: Mouse Heart Tissue, Rat Heart Tissue, HeLa Nuclear
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 37 kDa (predicted molecular weight: 39 kDa).
ICC/IF: Use a concentration of 5 µg/ml
IHC-P: 1/250
Target
Function
DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.
Tissue specificity
Ubiquitous.
Involvement in disease
Defects in OGG1 may be a cause of renal cell carcinoma (RCC) [MIM:144700]. It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
Sequence similarities
Belongs to the type-1 OGG1 family.
Cellular localization
Mitochondrion; Nucleus and Nucleus > nucleoplasm. Nucleus speckle. Nucleus matrix. Together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells.
Target information above from: UniProt accessionO15527
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 8 hydroxyguanine DNA glycosylase antibody
- 8 oxoguanine DNA glycosylase 1 antibody
- 8 oxoguanine DNA glycosylase antibody
see all
Database links
- Entrez Gene: 4968 Human
- Entrez Gene: 18294 Mouse
- Entrez Gene: 81528 Rat
- Omim: 601982 Human
- SwissProt: O15527 Human
- SwissProt: O08760 Mouse
- SwissProt: O70249 Rat
- Unigene: 380271 Human
see all
Anti-Ogg1 antibody images:
Western blot - Ogg1 antibody (ab22766)
Anti-Ogg1 antibody (ab22766) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab27251) at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 39 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Ogg1 antibody (ab22766)
ICC/IF image of ab22766 stained human HEK 293 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab22766, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HepG2 and MCF7 cells.
Western blot - Ogg1 antibody (ab22766)
All lanes : Anti-Ogg1 antibody (ab22766) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 39 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Exposure time : 8 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ogg1 antibody (ab22766)
ab22766 staining Ogg1 in Human osteosarcoma tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 5 minutes of peroxidase followed by 10 minutes of protein at 20°C; antigen retrieval was by heat mediation in target retrieval solution. The sample was incubated with primary antibody (1/500 in antibody diluent) at 20°C for 45 minutes. An undiluted HRP-conjugated polyclonal to rabbit IgG was used as secondary antibody.
This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
References for Anti-Ogg1 antibody (ab22766)
This product has been referenced in:
- Wang Jet al. Cardiac overexpression of 8-oxoguanine DNA glycosylase 1 protects mitochondrial DNA and reduces cardiac fibrosis following transaortic constriction. Am J Physiol Heart Circ Physiol 301:H2073-80 (2011).Read more (PubMed: 21873502) »
See 1 publication for this product
Publishing research using ab22766? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"