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Western blot - Ogg1 antibody (ab62826)
(enlarge)
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Product Name
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Ogg1 antibody
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See all Ogg1 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to Ogg1
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Immunogen
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Synthetic peptide: C-HPKTSQAKGPSPLANK, corresponding to internal sequence amino acids 282-297 of Rat Ogg1
BLAST 'C-HPKTSQAKGPSPLANK' with  or 
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Reacts with
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Rat
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Predicted to react
(species key)
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Ms
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/128000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 39 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Rat Spleen lysate
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Cellular localization
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Mitochondrial and Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
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Relevance
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The Ogg1 gene encodes a DNA repair enzyme (N glycosylase/DNA lyase) that incises DNA at 8 oxoG residues. N glycosylase/DNA lyase excises 7,8 dihydro 8 oxoguanine and 2,6 diamino 4 hydroxy 5 N methylformamidopyrimidine (FAPY) from damaged DNA. N glycosylase/DNA lyase has a beta lyase activity that nicks DNA 3' to the lesion.
Alternative splicing of the C terminal region of the Ogg1 gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N terminal region in common. Many alternative splice variants for this gene have been described, but the full length nature for every variant has not been determined. The N terminus of this gene contains a mitochondrial targetting signal, essential for mitochondrial localization.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Ogg1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Ogg1 antibody images, references, products related to ab62826 and other tools.
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Ogg1 antibody images:
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Search PubMed (MEDLINE) for references to Ogg1
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Ogg1 antibody - more information
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Customer reviews (feedback) regarding Ogg1 antibody |
Customer FAQs regarding Ogg1 antibody |
Protocols for Ogg1 antibody |
Price and availability of products related to Ogg1 antibody |
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Ogg1 antibody - related products:
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Ogg1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Ogg1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Western blot - Ogg1 antibody (ab62826)