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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Overview
Product name
Anti-Ogg1 antibody
See all Ogg1 products (7) ...
Description
Goat polyclonal to Ogg1
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Rat
Predicted to work with
Mouse, Cow, Human
Immunogen
Synthetic peptide: C-HPKTSQAKGPSPLANK, corresponding to internal sequence amino acids 282-297 of Rat Ogg1
C-HPKTSQAK GPSPLANK
Positive control
Rat Spleen lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
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Western blot - Ogg1 antibody (ab62826)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab62826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 0.3 µg/ml.Detects a band of approximately 37 kDa (predicted molecular weight: 39 kDa).
ELISA
ELISA: 1/128000ELISA: 1/128000
Target
Function
DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.
Tissue specificity
Ubiquitous.
Involvement in disease
Defects in OGG1 may be a cause of renal cell carcinoma (RCC) [MIM:144700]. It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
Sequence similarities
Belongs to the type-1 OGG1 family.
Cellular localization
Mitochondrion; Nucleus and Nucleus > nucleoplasm. Nucleus speckle. Nucleus matrix. Together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells.
Target information above from: UniProt accessionO15527
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 8 hydroxyguanine DNA glycosylase antibody
- 8 oxoguanine DNA glycosylase 1 antibody
- 8 oxoguanine DNA glycosylase antibody
see all
Database links
- Entrez Gene: 4968 Human
- Entrez Gene: 18294 Mouse
- Entrez Gene: 81528 Rat
- Omim: 601982 Human
- SwissProt: O15527 Human
- SwissProt: O08760 Mouse
- SwissProt: O70249 Rat
- Unigene: 380271 Human
see all
Anti-Ogg1 antibody images:
Western blot - Ogg1 antibody (ab62826)
Anti-Ogg1 antibody (ab62826) at 0.1 µg/ml + Rat Spleen lysate at 35 µg
Predicted band size : 39 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Detected by chemiluminescence
References for Anti-Ogg1 antibody (ab62826)
ab62826 has not yet been referenced specifically in any publications.
Publishing research using ab62826? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"