Overview

  • Product nameOil Red O (Lipid Stain)
  • Product overview

    Oil Red O (Lipid Stain) is intended for use in the histological visualization of fat cells and neutral fat. This kit may be used ONLY on frozen tissue sections, fresh smears, or touch preps.

  • Notes

    Staining Interpretation

      

    Fat Cells  Red
    Neutral Fat    Red
    NucleiBlue

     

    Control Tissue: Any frozen section containing fat.

  • Tested applicationsSuitable for: IHC-Frmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab150678 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr Use at an assay dependent concentration.

    Oil Red O (Lipid Stain) images

    • Ab150678 (Oil Red O stain) staining lipid deposits (red) in frozen normal human skin. The dermis is unstained, whereas the hypodermis is stained, demonstrating dye specificity. Hematoxylin (blue) is used as a counterstain.  

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • During adipocyte differentiation, cells accumulate lipid. Oil Red O staining kit (ab150678) was used to visualize lipid in 3T3-L1 cells (left) and adipocyte-like cells (right). As expected, lipid accumulates in 3T3-L1 cells after differentiation into adipocyte-like cells.

    Protocols

    References for Oil Red O (Lipid Stain) (ab150678)

    This product has been referenced in:
    • Jiao Y  et al. The Fto Gene Regulates the Proliferation and Differentiation of Pre-Adipocytes in Vitro. Nutrients 8:102 (2016). Read more (PubMed: 26907332) »
    • Patel AV  et al. Fatty acid synthase is a metabolic oncogene targetable in malignant peripheral nerve sheath tumors. Neuro Oncol 17:1599-608 (2015). Read more (PubMed: 26116612) »

    See all 3 Publications for this product

    Product Wall

    This reference contains a protocol for staining cultured cells with Oil Red O.

    Hope R. G., McLauchlan J. (2000) J. Gen. Virol. 81:1913–1925.

    http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-81-8-1913#tab2
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    I am sorry to confirm that we do not know if our Oil Red O solution will work for live adipocytes. We do not have a protocol or data for this and most of the protocols I have found require alcoholic solutions (ab150678 does not) and a fixatio...

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    The frozen tissues need to be fixed. Fixing in the PFA is fine. However, when fixing the tissue, avoid using reagents that will dissolve the fats.

    We would recommend putting about 200 to 300ul of the solution (depending on the size of the spec...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"