Recombinant Anti-Olig1 antibody [EPR6790] (ab124908)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6790] to Olig1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Olig1 antibody [EPR6790]
See all Olig1 primary antibodies -
Description
Rabbit monoclonal [EPR6790] to Olig1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MOLT4, SHSY-5Y, 293T and C6 cell lysates; 293T cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.40 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.1% BSA, 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6790 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 27 kDa).
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 27 kDa). |
Target
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Function
Promotes formation and maturation of oligodendrocytes, especially within the brain. Cooperates with OLIG2 to establish the pMN domain of the embryonic neural tube. -
Tissue specificity
Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas is highly variable. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 116448 Human
- Entrez Gene: 50914 Mouse
- Entrez Gene: 60394 Rat
- Omim: 606385 Human
- SwissProt: Q8TAK6 Human
- SwissProt: Q9JKN5 Mouse
- SwissProt: Q9WUQ3 Rat
- Unigene: 56663 Human
see all -
Alternative names
- Basic domain helix loop helix protein class B 6 antibody
- Basic domain helix loop helix protein class B6 antibody
- BHLH B6 antibody
see all
Images
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All lanes : Anti-Olig1 antibody [EPR6790] (ab124908) at 1/1000 dilution
Lane 1 : MOLT4 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 4 : C6 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted? -
Intracellular flow cytometric analysis of permeabilized 293T cells using ab124908 at 1/10 dilution (red) or a rabbit IgG negative control (green).
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab124908 has been referenced in 2 publications.
- Jiang H et al. Amelioration of experimental autoimmune encephalomyelitis through transplantation of placental derived mesenchymal stem cells. Sci Rep 7:41837 (2017). PubMed: 28186117
- Cheng T et al. Effect of OLIG1 on the development of oligodendrocytes and myelination in a neonatal rat PVL model induced by hypoxia-ischemia. Mol Med Rep 11:2379-86 (2015). WB, IHC ; Rat . PubMed: 25435330