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ab111666 |
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Dear sir or madam, |
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ANSWER: |
Thank you very much for contacting us. |
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Please can you let me know the immunoigen sequence - I want to compare with zebrafish to see if there is a good chance of cross-reactivity?
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ANSWER: |
Thank you for your patience in this matter. I have received a reply from the originator of ab42453 (Olig2 antibody). The exact immunogenic peptide sequence is proprietary information. However, we can state that it is within the following 50 amino acid sequence: SLPGSGLPSVGSIRPPHGLLKSPSAAAAAPLGGGGGGSGASGGFQHWGGM I hope this information helps. Please don't hesitate to contact us again if you have further questions. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-Olig2 antibody (ab42453) at 0.5 µg/ml (in 5% skim milk/PBS buffer) + Jurkat cell lysate
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 32 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Additional bands at : 28 kDa,38 kDa. We are unsure as to the identity of these extra bands.
ab42453 at a 1/100 dilution staining Olig2 in human brain tissue (pontine base) by Immunohistochemistry (frozen sections) incubated for 1 hour at 20°C. Acetone fixed. Blocked with 10% milk for 30 minutes at 20°C. Secondary used at 1/200 pig polyclonal conjugated to biotin.
Note nuclear and cytoplasmic staining of oligodendrocytes in the white matter and neurons in the grey matter.
This image is from an anonymous abreview.
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