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Products:Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Overview
Product name
Anti-Olig2 antibody
See all Olig2 products (8) ...
Description
Rabbit polyclonal to Olig2
Tested applications
WB, ICC/IF, IHC-Fr, IHC (PFA fixed)more details
Cross reactivity
Reacts with
Human, Rhesus monkey
Predicted to work with
Mouse, Chicken
Immunogen
A region within synthetic peptide: SLPGSGLPSV GSIRPPHGLL KSPSAAAAAP LGGGGGGSGA SGGFQHWGGM, corresponding to amino acids 242-291 of Olig2
SLPGSGLPSV GSIRPPHGLL KSPSAAAAAP LGGGGGGSGA SGGFQHWGGM
Positive control
Jurkat cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Constituent: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab42453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 µg/mlDetects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
ICC/IF: Use at an assay dependent concentration. (PubMed: 20025521)
IHC-Fr: 1/200( (see Abreview and notes))
IHC (PFA fixed)
IHC (PFA fixed): 1/1000IHC (PFA fixed): 1/1000
Target
Function
Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
Tissue specificity
Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
Involvement in disease
Note=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Domain
The bHLH is essential for interaction with NKX2-2.
Cellular localization
Nucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2.
Target information above from: UniProt accessionQ13516
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Basic domain helix loop helix protein class B 1 antibody
- Basic helix loop helix protein class B 1 antibody
- BHLHB1 antibody
see all
Database links
- Entrez Gene: 428612 Chicken
- Entrez Gene: 10215 Human
- Entrez Gene: 50913 Mouse
- Omim: 606386 Human
- SwissProt: Q13516 Human
- SwissProt: Q9EQW6 Mouse
- Unigene: 176977 Human
- Unigene: 37289 Mouse
Anti-Olig2 antibody images:
Western blot - Olig2 antibody (ab42453)
Anti-Olig2 antibody (ab42453) at 0.5 µg/ml (in 5% skim milk/PBS buffer) + Jurkat cell lysate
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 32 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Additional bands at : 28 kDa,38 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Frozen sections) - Olig2 antibody (ab42453)
ab42453 at a 1/100 dilution staining Olig2 in human brain tissue (pontine base) by Immunohistochemistry (frozen sections) incubated for 1 hour at 20°C. Acetone fixed. Blocked with 10% milk for 30 minutes at 20°C. Secondary used at 1/200 pig polyclonal conjugated to biotin.
Note nuclear and cytoplasmic staining of oligodendrocytes in the white matter and neurons in the grey matter.
This image is from an anonymous abreview.
References for Anti-Olig2 antibody (ab42453)
This product has been referenced in:
- Sun Tet al. Evidence for motoneuron lineage-specific regulation of Olig2 in the vertebrate neural tube. Dev Biol 292:152-64 (2006).Read more (PubMed: 16469306) »
- Wang Ket al. Dynamic epigenetic regulation of the Oct4 and Nanog regulatory regions during neural differentiation in rhesus nuclear transfer embryonic stem cells. Cloning Stem Cells 11:483-96 (2009). ICC/IF; Rhesus monkey.Read more (PubMed: 20025521) »
See all 3 publications for this product
Publishing research using ab42453? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"