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Western blot - Olig2 antibody (ab56643)
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Product Name
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Olig2 antibody
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See all Olig2 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to Olig2
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Immunogen
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Recombinant full length protein: DSDASLVSSR PSSPEPDDLF LPARSKGSSG SAFTGGTVSS STPSDCPPEL SAELRGAMGS AGAHPGDKLG GSGFKSS, corresponding to amino acids 2-78 of Human Olig2
BLAST 'DSDASLVSSR PSSPEPDDLF LPARSKGSSG SAFTGGTVSS STPSDCPPEL SAELRGAMGS AGAHPGDKLG ' with  or 
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Reacts with
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear and cytoplasmic. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2.
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Research areas
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Stem Cells >> Neural Stem Cells >> Neuron Restricted Lineage
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
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Relevance
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Olig2 (oligodendrocyte transcription factor 2) is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. It cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. It is an antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Olig2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Olig2 antibody images, references, products related to ab56643 and other tools.
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Olig2 antibody images:
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 Western blot - Olig2 antibody (ab56643)
Western blot against tagged recombinant protein immunogen using ab56643 Olig2 antibody at 1ug/ml. Predicted band size of immunogen is 35 kDa
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Search PubMed (MEDLINE) for references to Olig2
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Olig2 antibody - more information
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Customer reviews (feedback) regarding Olig2 antibody |
Customer FAQs regarding Olig2 antibody |
Protocols for Olig2 antibody |
Price and availability of products related to Olig2 antibody |
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Olig2 antibody - related products:
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Olig2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Olig2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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