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Products:Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Overview
Product name
Anti-Olig2 antibody
See all Olig2 products (8) ...
Description
Rabbit polyclonal to Olig2
Tested applications
ICC/IF, WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide from the N terminal region of human Olig2, conjugated to an immunogenic carrier protein. The antigen is homologous in rat and mouse.
Positive control
Mouse brain lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab81093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: 1/1000Predicted molecular weight: 32 kDa.(Block with 1% LFDM for 15 minutes at room temperature, incubate primary antibody for 15 minutes at room temperature, wash 3 times with PBST, 5 minutes each. )
IHC-P: 1/1000 - 1/3000.Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.(AR buffer: Citrate buffer, pH 6 containing 0.05% Tween-20 preheat: 65C cycle: 98C for 25min cool down: 37C)
IHC-Fr: Use at an assay dependent dilution. (PubMed: 20596532)
Target
Function
Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
Tissue specificity
Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
Involvement in disease
Note=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Domain
The bHLH is essential for interaction with NKX2-2.
Cellular localization
Nucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2.
Target information above from: UniProt accessionQ13516
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Basic domain helix loop helix protein class B 1 antibody
- Basic helix loop helix protein class B 1 antibody
- bHLHB1 antibody
see all
Database links
- Entrez Gene: 10215 Human
- Entrez Gene: 50913 Mouse
- Entrez Gene: 304103 Rat
- Omim: 606386 Human
- SwissProt: Q13516 Human
- SwissProt: Q9EQW6 Mouse
- Unigene: 176977 Human
- Unigene: 37289 Mouse
see all
Anti-Olig2 antibody images:
Western blot - Olig2 antibody (ab81093)
Anti-Olig2 antibody (ab81093) at 1/1000 dilution + mouse brain lysate (denatured, reduced)
Predicted band size : 32 kDa
Observed band size : 32 kDa
The blocking was performed with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBS (1x), 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Olig2 antibody(ab81093)
IHC image of ab81093 staining in human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab81093, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody (ab81093)
ICC/IF image of ab81093 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81093, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Olig2 antibody (ab81093)
This product has been referenced in:
- Hosking MPet al. CXCR2 signaling protects oligodendrocytes and restricts demyelination in a mouse model of viral-induced demyelination. PLoS One 5:e11340 (2010). IHC-Fr; Mouse.Read more (PubMed: 20596532) »
See 1 publication for this product
Publishing research using ab81093? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"