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Product Name 

Olig3 antibody

 

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Product type 

Primary antibodies

Description 

Rabbit polyclonal to Olig3

Immunogen 

Synthetic peptide, corresponding to amino acids 53-66 of Human Olig3

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

ICC, IHC-Fr, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ICC: 1/200 - 1/1000.
IHC-Fr: 1/200 - 1/1000 for 90 minutes. Cut 10um slices on a cryostat and dry slides at RT for 30-90 minutes. Fix with cold Acetone/Methanol (50/50) for 2 minutes or alternatively with 4% PFA followed by treatment with a 0.05% trypsin solution (incubate for 10 minutes at 37°C, then wash 3 times with PBS)
WB: 1/200 - 1/1000. Predicted molecular weight: 29 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Neuroscience >> Neurology process >> Neurogenesis

Relevance 

The Olig family is a sub-family of the basic helix-loop-helix transcription factors. Olig1 and Olig2, which have been implicated in oligodendrogenesis, are expressed in the region of the ventral ventricular zone of late embryonic spinal cord where oligodendrocyte progenitors appear. Olig3 is transiently expressed in different types of progenitors of embryonic central nervous system and then disappears in the course of development. In adult, expression of Olig3 is primarily detected in non-neural tissues.

 

Alternative names Olig3 antibody (ab78006)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

167826    (Human)
94222    (Mouse)

Omim

    

609323    (Human)

SwissProt

    

Q7RTU3    (Human)
Q6PFG8    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 1% BSA, Ammonium sulphate, PBS

Purification notes 

Antibody is affinity purified but presented as an ammonium sulfate precipitated purified material for stability.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Olig3 antibody is on this datasheet. But please do contact us if you would like any reassurance!


 

Search PubMed (MEDLINE) for references to Olig3

 

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