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Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-Olig3 antibody
See all Olig3 products (5) ...
Description
Rabbit polyclonal to Olig3
Tested applications
IHC-Fr, WB, ICCmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide, corresponding to amino acids 53-66 of Human Olig3
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Constituents: 50% Ammonium sulphate, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
Antibody is affinity purified but presented as an ammonium sulfate precipitated purified material for stability.
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab78006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr
WB
ICC
Application notes
ICC: 1/200 - 1/1000.
IHC-Fr: 1/200 - 1/1000 for 90 minutes. Cut 10um slices on a cryostat and dry slides at RT for 30-90 minutes. Fix with cold Acetone/Methanol (50/50) for 2 minutes or alternatively with 4% PFA followed by treatment with a 0.05% trypsin solution (incubate for 10 minutes at 37°C, then wash 3 times with PBS)
WB: 1/200 - 1/1000. Predicted molecular weight: 29 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ7RTU3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Basic helix loop helix domain containing class B protein 7 antibody
- Bhlhb7 antibody
- bHLHe20 antibody
see all
Database links
- Entrez Gene: 167826 Human
- Entrez Gene: 94222 Mouse
- Entrez Gene: 293012 Rat
- Omim: 609323 Human
- SwissProt: Q7RTU3 Human
- SwissProt: Q6PFG8 Mouse
- SwissProt: D4A572 Rat
- Unigene: 195398 Human
see all
References for Anti-Olig3 antibody (ab78006)
ab78006 has not yet been referenced specifically in any publications.
Publishing research using ab78006? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"