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Product Name
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Olig3 antibody
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See all Olig3 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Olig3
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Immunogen
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Synthetic peptide, corresponding to amino acids 53-66 of Human Olig3
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ICC, IHC-Fr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ICC: 1/200 - 1/1000. IHC-Fr: 1/200 - 1/1000 for 90 minutes. Cut 10um slices on a cryostat and dry slides at RT for 30-90 minutes. Fix with cold Acetone/Methanol (50/50) for 2 minutes or alternatively with 4% PFA followed by treatment with a 0.05% trypsin solution (incubate for 10 minutes at 37°C, then wash 3 times with PBS) WB: 1/200 - 1/1000. Predicted molecular weight: 29 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Neuroscience >> Neurology process >> Neurogenesis
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Relevance
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The Olig family is a sub-family of the basic helix-loop-helix transcription factors. Olig1 and Olig2, which have been implicated in oligodendrogenesis, are expressed in the region of the ventral ventricular zone of late embryonic spinal cord where oligodendrocyte progenitors appear. Olig3 is transiently expressed in different types of progenitors of embryonic central nervous system and then disappears in the course of development. In adult, expression of Olig3 is primarily detected in non-neural tissues.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None Constituents: 1% BSA, Ammonium sulphate, PBS
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Purification notes
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Antibody is affinity purified but presented as an ammonium sulfate precipitated purified material for stability.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Olig3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Olig3
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Olig3 antibody - more information
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