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Products:Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Overview
Product name
Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker
See all Oligodendrocyte Specific Protein products (5) ...
Description
Rabbit polyclonal to Oligodendrocyte Specific Protein - Oligodendrocyte Marker
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Horse, Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Oligodendrocyte Specific Protein OSP.
(Peptide available as ab66447.)
Positive control
This antibody gave a positive signal in the following Tissue Lysates: Human Heart Human Colon Human Small Intestine Rat Spinal Cord Rat Testis Mouse Skeletal Muscle (data not shown) Rat Spleen (data not shown)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Immunology >> Immune System Diseases >> Autoimmune
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
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Western blot - Oligodendrocyte Specific Protein OSP antibody (ab64369)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab64369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Application notes
Is unsuitable for or ICC/IF.
Target
Relevance
Oligodendrocyte specific protein (OSP), also known Claudin 11, is the third most abundant component of central nervous system myelin. The claudin superfamily consists of many structurally related proteins. These proteins, which include claudin 1 through 18, are located in both epithelial and endothelial cells in all tight junction bearing tissues. Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. There is growing evidence that OSP/Claudin 11 determines the permeability between layers of myelin sheaths via focal adhesion. Its expression is highly regulated during development, suggesting that it may play an important role in the growth and differentiation of oligodendrocytes and other cells outside the CNS. In addition, the protein is a candidate autoantigen in the development of autoimmune demyelinating disease.
Cellular localization
Integral cell membrane protein. Tight junctions.
Alternative names
- Claudin 11 antibody
- claudin11 antibody
- CLDN 11 antibody
see all
Database links
- Entrez Gene: 5010 Human
- Entrez Gene: 18417 Mouse
- Entrez Gene: 84588 Rat
- Omim: 601326 Human
- SwissProt: O75508 Human
- SwissProt: Q60771 Mouse
- SwissProt: Q99P82 Rat
- Unigene: 31595 Human
see all
Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker images:
Western blot - Oligodendrocyte Specific Protein OSP antibody (ab64369)
All lanes : Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab64369) at 1 µg/ml
Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
Lane 2 : Spinal Cord (Rat) Tissue Lysate
Lane 3 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)
Lane 4 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)
Lane 5 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 22 kDa
Observed band size : 22 kDa
Additional bands at : 52 kDa,60 kDa,70 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab64369)
ab64369 has not yet been referenced specifically in any publications.
Publishing research using ab64369? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"