Immunocytochemistry/ Immunofluorescence - Oligodendrocyte Specific Protein antibody (ab53041)

ab53041 staining Oligodendrocyte Specific Protein in mixed glia prepared from mouse brain by Immunocytochemistry/ Immunofluorescence. The cells were fixed in ice-cold methanol and then blocked using 20% serum with 0.5% Saponin for 1 hour at 4ºC. Samples were then incubated with primary antibody at 1/200 for 2 hours at 23ºC. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/400 dilution. DAPI was used to stain the cell nuclei (blue).

Image courtesy of an anonymous Abreview.

Western blot - Oligodendrocyte Specific Protein OSP antibody (ab53041)

All lanes : Anti-Oligodendrocyte Specific Protein antibody (ab53041) at 1/500 dilution

Lane 1 : Mouse brain extract
Lane 2 : Mouse brain extract with peptide


Predicted band size : 22 kDa
Observed band size : 22 kDa

Immunohistochemistry (Frozen sections) - Oligodendrocyte Specific Protein OSP antibody (ab53041)

ab53041 diluted 1/500 in 2% normal goat serum staining rat brain tissue sections by IHC-Fr. The tissue was formaldehyde fixed and blocked with 2% serum for 30 minutes at 21°C before incubation with the primary antibody for 24 hours at 21°C.  A biotinylated goat anti-rabbit antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Dr J Moffett

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Oligodendrocyte Specific Protein antibody (ab53041)

ab53041 staining Oligodendrocyte Specific Protein in mouse brain tissue section by Immunohistochemistry (PFA-fixed). The tissue sections were fixed with 4% paraformaldehyde.The primary antibody was diluted 1/3000 and incubated with sample for 16 hours at 4°C in PBS 0.3% Triton 8% BSA. An Alexa Fluor® 568 conjugated goat polyclonal to rabbit IgG was used at dilution 1/1000 as secondary antibody. The image was taken from the cortex of an adult mouse, near the midline.

This image is a courtesy of Anonymous Abreview

Immunocytochemistry/ Immunofluorescence - Oligodendrocyte Specific Protein antibody (ab53041)

ICC/IF image of ab53041 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53041, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody (ab53041)

ab53041 staining cat brain sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab53041 at 1/500 in TRIS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Carl Hobbs