Feedback Form

Western blot - Oligodendrocyte Specific Protein OSP antibody (ab53041)
Western blot - Oligodendrocyte Specific Protein OSP antibody (ab53041)
(enlarge)


Immunohistochemistry (Frozen sections) - Oligodendrocyte Specific Protein OSP antibody (ab53041)
Immunohistochemistry (Frozen sections) - Oligodendrocyte Specific Protein OSP antibody (ab53041)
(enlarge)


Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Oligodendrocyte Specific Protein antibody (ab53041)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Oligodendrocyte Specific Protein antibody (ab53041)
(enlarge)


Product Name 

Oligodendrocyte Specific Protein antibody

 

See all Oligodendrocyte Specific Protein antibodies (5)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Oligodendrocyte Specific Protein

Immunogen 

Synthetic peptide (Human)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Detects endogenous levels of total Oligodendrocyte Specific Protein

Tested applications 
(see key)

ELISA, IHC (PFA fixed), IHC-Fr, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
(Read all Abreviews)

Submit an Abreview and earn up to 220 Abpoints by reviewing this product

• IHC-Fr

1 Abreview

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

• IHC (PFA fixed)

1 Abreview

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)


IHC (PFA fixed)
(read Abreview)


IHC-Fr
(read Abreview)


Application notes 
(see key)

Recommended dilutions
ELISA: 1/5000.
IHC-Fr: Use at an assay dependent dilution.
IHC (PFA fixed): 1/3000.
WB: 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mouse brain extract

Cellular localization 

Integral cell membrane protein. Tight junctions.

Research areas 

Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Immunology >> Immune System Diseases >> Autoimmune
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker

Relevance 

Oligodendrocyte specific protein (OSP), also known Claudin 11, is the third most abundant component of central nervous system myelin. The claudin superfamily consists of many structurally related proteins. These proteins, which include claudin 1 through 18, are located in both epithelial and endothelial cells in all tight junction bearing tissues. Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. There is growing evidence that OSP/Claudin 11 determines the permeability between layers of myelin sheaths via focal adhesion. Its expression is highly regulated during development, suggesting that it may play an important role in the growth and differentiation of oligodendrocytes and other cells outside the CNS. In addition, the protein is a candidate autoantigen in the development of autoimmune demyelinating disease.

 

Oligodendrocyte Specific Protein antibody (ab53041)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

5010    (Human)
18417    (Mouse)
84588    (Rat)

Omim

    

601326    (Human)

SwissProt

    

O75508    (Human)
Q60771    (Mouse)
Q99P82    (Rat)

Unigene

    

31595    (Human)
4425    (Mouse)
8282    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Oligodendrocyte Specific Protein antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Oligodendrocyte Specific Protein antibody images, references, products related to ab53041 and other tools.

 

Oligodendrocyte Specific Protein antibody images:

Product image  Western blot - Oligodendrocyte Specific Protein OSP antibody (ab53041)

Western blot - Oligodendrocyte Specific Protein OSP antibody (ab53041)

All lanes : Oligodendrocyte Specific Protein antibody (ab53041) at 1/500 dilution

Lane 1 : Mouse brain extract
Lane 2 : Mouse brain extract with peptide


Predicted band size : 22 kDa
Observed band size : 22 kDa

 

Product image  Immunohistochemistry (Frozen sections) - Oligodendrocyte Specific Protein OSP antibody (ab53041)

Immunohistochemistry (Frozen sections) - Oligodendrocyte Specific Protein OSP antibody (ab53041)

ab53041 diluted 1/500 in 2% normal goat serum staining rat brain tissue sections by IHC-Fr. The tissue was formaldehyde fixed and blocked with 2% serum for 30 minutes at 21°C before incubation with the primary antibody for 24 hours at 21°C.  A biotinylated goat anti-rabbit antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Dr J Moffett

See Abreview

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Oligodendrocyte Specific Protein antibody (ab53041)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Oligodendrocyte Specific Protein antibody (ab53041)

ab53041 staining Oligodendrocyte Specific Protein in mouse brain tissue section by Immunohistochemistry (PFA-fixed). The tissue sections were fixed with 4% paraformaldehyde.The primary antibody was diluted 1/3000 and incubated with sample for 16 hours at 4°C in PBS 0.3% Triton 8% BSA. An Alexa Fluor® 568 conjugated goat polyclonal to rabbit IgG was used in 1/1000 dilution, as secondary. The image is taken from the cortex of an adult mouse, near the midline.

This image is a courtesy of Anonimous Abreview

See Abreview

 
 

Search PubMed (MEDLINE) for references to Oligodendrocyte Specific Protein

 

Oligodendrocyte Specific Protein antibody - more information

Customer reviews (feedback) regarding Oligodendrocyte Specific Protein antibody

Customer FAQs regarding Oligodendrocyte Specific Protein antibody

Protocols for Oligodendrocyte Specific Protein antibody

Price and availability of products related to Oligodendrocyte Specific Protein antibody

 

Oligodendrocyte Specific Protein antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for Oligodendrocyte Specific Protein antibody

ab30149

  

Brain (Mouse) Membrane Lysate - normal tissue

 

Compatible Secondaries for Oligodendrocyte Specific Protein antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other Oligodendrocyte Specific Protein Antibodies

See all Oligodendrocyte Specific Protein antibodies (5)...

 

Oligodendrocyte Specific Protein antibody - other tools:

Contact Abcam with a Technical Enquiry about Oligodendrocyte Specific Protein antibody

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.