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Read our guarantee »Products:Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Anti-Oligodendrocyte Specific Protein antibody
See all Oligodendrocyte Specific Protein products (5) ...
Rabbit polyclonal to Oligodendrocyte Specific Protein
Detects endogenous levels of total Oligodendrocyte Specific Protein
IHC-Fr, ICC/IF, WB, ELISA, IHC-P, IHC (PFA fixed)more details
Reacts with
Mouse, Rat, Sheep, Goat, Cow, Cat, Human, Marmoset (common)
Synthetic peptide (Human)
Mouse brain extract
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Immunology >> Immune System Diseases >> Autoimmune
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Our Abpromise guarantee covers the use of ab53041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
ICC/IF: Use a concentration of 1 µg/ml
WB: 1/500 - 1/1000.Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ELISA: 1/5000
IHC-P: 1/500Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC (PFA fixed): 1/3000
Oligodendrocyte specific protein (OSP), also known Claudin 11, is the third most abundant component of central nervous system myelin. The claudin superfamily consists of many structurally related proteins. These proteins, which include claudin 1 through 18, are located in both epithelial and endothelial cells in all tight junction bearing tissues. Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. There is growing evidence that OSP/Claudin 11 determines the permeability between layers of myelin sheaths via focal adhesion. Its expression is highly regulated during development, suggesting that it may play an important role in the growth and differentiation of oligodendrocytes and other cells outside the CNS. In addition, the protein is a candidate autoantigen in the development of autoimmune demyelinating disease.
Integral cell membrane protein. Tight junctions.
Immunocytochemistry/ Immunofluorescence - Oligodendrocyte Specific Protein antibody (ab53041)

ab53041 staining Oligodendrocyte Specific Protein in mixed glia prepared from mouse brain by Immunocytochemistry/ Immunofluorescence. The cells were fixed in ice-cold methanol and then blocked using 20% serum with 0.5% Saponin for 1 hour at 4°C. Samples were then incubated with primary antibody at 1/200 for 2 hours at 23°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/400 dilution. DAPI was used to stain the cell nuclei (blue).
Image courtesy of an anonymous Abreview.
Western blot - Oligodendrocyte Specific Protein OSP antibody (ab53041)

All lanes : Anti-Oligodendrocyte Specific Protein antibody (ab53041) at 1/500 dilution
Lane 1 : Mouse brain extract
Lane 2 : Mouse brain extract with peptide
Predicted band size : 22 kDa
Observed band size : 22 kDa
Immunohistochemistry (Frozen sections) - Oligodendrocyte Specific Protein OSP antibody (ab53041)

ab53041 diluted 1/500 in 2% normal goat serum staining rat brain tissue sections by IHC-Fr. The tissue was formaldehyde fixed and blocked with 2% serum for 30 minutes at 21°C before incubation with the primary antibody for 24 hours at 21°C. A biotinylated goat anti-rabbit antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Dr J Moffett
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Oligodendrocyte Specific Protein antibody (ab53041)

ab53041 staining Oligodendrocyte Specific Protein in mouse brain tissue section by Immunohistochemistry (PFA-fixed). The tissue sections were fixed with 4% paraformaldehyde.The primary antibody was diluted 1/3000 and incubated with sample for 16 hours at 4°C in PBS 0.3% Triton 8% BSA. An Alexa Fluor® 568 conjugated goat polyclonal to rabbit IgG was used at dilution 1/1000 as secondary antibody. The image was taken from the cortex of an adult mouse, near the midline.
This image is a courtesy of Anonymous Abreview
Immunocytochemistry/ Immunofluorescence - Oligodendrocyte Specific Protein antibody (ab53041)

ICC/IF image of ab53041 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53041, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody (ab53041)

ab53041 staining cat brain sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab53041 at 1/500 in TRIS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Carl Hobbs
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ab53041 staining Oligodendrocyte Specific Protein in mixed glia prepared from mouse brain by Immunocytochemistry/ Immunofluorescence. The cells were fixed in ice-cold methanol and then blocked using 20% serum with 0.5% Saponin for 1 hour at 4°C. Samples were then incubated with primary antibody at 1/200 for 2 hours at 23°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/400 dilution. DAPI was used to stain the cell nuclei (blue).
Image courtesy of an anonymous Abreview.

All lanes : Anti-Oligodendrocyte Specific Protein antibody (ab53041) at 1/500 dilution
Lane 1 : Mouse brain extract
Lane 2 : Mouse brain extract with peptide
Predicted band size : 22 kDa
Observed band size : 22 kDa

ab53041 diluted 1/500 in 2% normal goat serum staining rat brain tissue sections by IHC-Fr. The tissue was formaldehyde fixed and blocked with 2% serum for 30 minutes at 21°C before incubation with the primary antibody for 24 hours at 21°C. A biotinylated goat anti-rabbit antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Dr J Moffett

ab53041 staining Oligodendrocyte Specific Protein in mouse brain tissue section by Immunohistochemistry (PFA-fixed). The tissue sections were fixed with 4% paraformaldehyde.The primary antibody was diluted 1/3000 and incubated with sample for 16 hours at 4°C in PBS 0.3% Triton 8% BSA. An Alexa Fluor® 568 conjugated goat polyclonal to rabbit IgG was used at dilution 1/1000 as secondary antibody. The image was taken from the cortex of an adult mouse, near the midline.
This image is a courtesy of Anonymous Abreview

ICC/IF image of ab53041 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53041, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab53041 staining cat brain sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab53041 at 1/500 in TRIS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Carl Hobbs








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