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Western blot - Oligodendrocyte Specific Protein OSP antibody (ab53041)
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Immunohistochemistry (Frozen sections) - Oligodendrocyte Specific Protein OSP antibody (ab53041)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Oligodendrocyte Specific Protein antibody (ab53041)
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Product Name
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Oligodendrocyte Specific Protein antibody
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See all Oligodendrocyte Specific Protein antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Oligodendrocyte Specific Protein
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Immunogen
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Synthetic peptide (Human)
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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Detects endogenous levels of total Oligodendrocyte Specific Protein
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Tested applications
(see key)
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ELISA, IHC (PFA fixed), IHC-Fr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/5000.
IHC-Fr: Use at an assay dependent dilution.
IHC (PFA fixed): 1/3000.
WB: 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
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Mouse brain extract
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Cellular localization
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Integral cell membrane protein. Tight junctions.
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Research areas
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Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Immunology >> Immune System Diseases >> Autoimmune
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
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Relevance
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Oligodendrocyte specific protein (OSP), also known Claudin 11, is the third most abundant component of central nervous system myelin. The claudin superfamily consists of many structurally related proteins. These proteins, which include claudin 1 through 18, are located in both epithelial and endothelial cells in all tight junction bearing tissues. Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. There is growing evidence that OSP/Claudin 11 determines the permeability between layers of myelin sheaths via focal adhesion. Its expression is highly regulated during development, suggesting that it may play an important role in the growth and differentiation of oligodendrocytes and other cells outside the CNS. In addition, the protein is a candidate autoantigen in the development of autoimmune demyelinating disease.
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Oligodendrocyte Specific Protein antibody (ab53041) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Oligodendrocyte Specific Protein antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Oligodendrocyte Specific Protein antibody images, references, products related to ab53041 and other tools.
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Oligodendrocyte Specific Protein antibody images:
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 Western blot - Oligodendrocyte Specific Protein OSP antibody (ab53041)
All lanes : Oligodendrocyte Specific Protein antibody (ab53041) at 1/500 dilution
Lane 1 : Mouse brain extract
Lane 2 : Mouse brain extract with peptide
Predicted band size : 22 kDa
Observed band size : 22 kDa
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Immunohistochemistry (Frozen sections) - Oligodendrocyte Specific Protein OSP antibody (ab53041)
ab53041 diluted 1/500 in 2% normal goat serum staining rat brain tissue sections by IHC-Fr. The tissue was formaldehyde fixed and blocked with 2% serum for 30 minutes at 21°C before incubation with the primary antibody for 24 hours at 21°C. A biotinylated goat anti-rabbit antibody was used as the secondary. This image is courtesy of an Abreview submitted by Dr J Moffett
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Oligodendrocyte Specific Protein antibody (ab53041)
ab53041 staining Oligodendrocyte Specific Protein in mouse brain tissue section by Immunohistochemistry (PFA-fixed). The tissue sections were fixed with 4% paraformaldehyde.The primary antibody was diluted 1/3000 and incubated with sample for 16 hours at 4°C in PBS 0.3% Triton 8% BSA. An Alexa Fluor® 568 conjugated goat polyclonal to rabbit IgG was used in 1/1000 dilution, as secondary. The image is taken from the cortex of an adult mouse, near the midline. This image is a courtesy of Anonimous Abreview
See Abreview
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Search PubMed (MEDLINE) for references to Oligodendrocyte Specific Protein
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Oligodendrocyte Specific Protein antibody - more information
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Customer reviews (feedback) regarding Oligodendrocyte Specific Protein antibody |
Customer FAQs regarding Oligodendrocyte Specific Protein antibody |
Protocols for Oligodendrocyte Specific Protein antibody |
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Oligodendrocyte Specific Protein antibody - related products:
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Oligodendrocyte Specific Protein antibody - other tools:
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Contact Abcam with a Technical Enquiry about Oligodendrocyte Specific Protein antibody |
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