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Highly pure (>98%) recombinant Human Oncostatin M.
Our Abpromise guarantee covers the use of ab110621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Oncostatin M.|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Oncostatin M (Biotin) (ab84256). Using ab84256 as the detection antibody and ab110621 as the capture antibody, the detection limit for recombinant Human Oncostatin M is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 28 kDa. The detection limit for recombinant Human Oncostatin M is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|Neutralising||Use a concentration of 0.1 - 0.175 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of Oncostatin M (10.0 ng/ml)|
ab110621 has not yet been referenced specifically in any publications.