Overview

  • Product nameAnti-Oncostatin M antibodySee all Oncostatin M primary antibodies ...
  • Description
    Rabbit polyclonal to Oncostatin M
  • Tested applicationsIndirect ELISA, Sandwich ELISA, WB, Neutralising more details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Highly pure (>98%) recombinant Human Oncostatin M.

  • Positive control
    • ab110621 was purified by affinity chromatography employing immobilized Human Oncostatin M matrix.

Properties

Applications

Our Abpromise guarantee covers the use of ab110621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Oncostatin M.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Oncostatin M (Biotin) (ab84256). Using ab84256 as the detection antibody and ab110621 as the capture antibody, the detection limit for recombinant Human Oncostatin M is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 28 kDa. The detection limit for recombinant Human Oncostatin M is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralising Use a concentration of 0.1 - 0.175 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of Oncostatin M (10.0 ng/ml)

Target

  • FunctionGrowth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST).
  • Sequence similaritiesBelongs to the LIF/OSM family.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P13725 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • MGC20461 antibody
    • ONCM_HUMAN antibody
    • Oncostatin M antibody
    • Oncostatin-M antibody
    • OSM antibody
    see all

References for Anti-Oncostatin M antibody (ab110621)

ab110621 has not yet been referenced specifically in any publications.

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