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Overview

  • Product nameOptiblot Blue
  • General notesOptiblot Blue is a single step Coomassie based gel stain that is specially formulated for ultra-fast, sensitive and safe detection of your proteins. Protein gels can be stained in minutes without the need to wash, fix or destain. Only proteins are stained resulting in well defined blue bands on a highly transparent background. The reduction of background interference results in a better signal to noise ratio and may also have a positive impact on the overall resolution and sensitivity. The Optiblot Blue formulation is non-toxic and does not contain any methanol. Proteins stained using the Optiblot Blue stain are also compatible with mass spectrometry (MS) analysis.


    Manufactured under patent numbers : US 12/226,797 ; SG 201103010-3 ; SG 200807953-5 ; JP 2009-507182 ; IN 5722/CHENP/2008 ; GB 0608377.8 ; EP 10187154.9; EP 07733647.7 ; CN 200780021434.8 ; CA 2,649,835 ; AU 2007245443

    • Properties

  • Storage instructionsStore at +4°C.
  • Storage bufferConstituents: 2% Ethanol, 12% Phosphoric acid, 0.05% Coomassie Brilliant Blue
    Note: 5% solubilisers
  • Concentration information loading...
  • Research Areas
    • Alternative names
        Coomassie BlueCoomassie Brilliant BlueCoomassie G-250

    Optiblot Blue images

    • - Optiblot Blue (ab119211)
      - Optiblot Blue (ab119211)Image courtesy of anonymous abreview
      SDS-PAGE image showing molecular weight marker (MW) and His-tag recombinant protein fractions purified in Ni-NTA resin in lanes 1-5 stained with Optiblot Blue. Optiblot Blue was easy to handle and use - just pour a small amount onto your gel and within 10 minutes you can see the protein bands! No methanol and glacial acetic acid - no smell!

    Protocols

    References for Optiblot Blue (ab119211)

    ab119211 has not yet been referenced specifically in any publications.

    Publishing research using ab119211 ? Please let us know so that we can cite the reference in this datasheet

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    I am looking for loading control for mouse heart protein on western blotting. What is the appropriate antibody I should purchase? Thank you.


    The loading control should have a different molecular weight than the target protein(s) that you are testing for. The two most common loading controls are beta actin and GAPDH. I've included links below to some of our most highly reviewed and referen...

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    Could you tell me the epitope of the VWF antibodies listed in the subject line.  Specifically, I am looking for a VWF antibody that I could use in multiple applications (WB, ELISA, and IP ideally) and that recognizes an epitope on the N-terminal of VWF.  ...

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    Thank you for contacting us.

    There is very little epitope information about any of the Von Willebrand Factor antibodies; because of this few have been identified as recognizing the N-terminus of vWF. Some of them may recognize the N-term, bu...

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    I would like to try the ab218999 for immunoprecipitation, which is the working dilution range for this assay? Thanks,

    Thank you for contacting us.

    When using ab21899 Anti-beta 2 Microglobulin antibody [B2M-01] (Biotin) in IP we can recommend a working range of 1-10 ug/ml. The exact concentration for your IP experiments should be within this range but could b...

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    Dear tech:



    I want to know what is type of Coomassie Brilliant blue in Optiblot blue (ab119211), R250 or G250?



    Thank you for contacting us.

    Optiblot Blue is a G250 based stain.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Dear Abcam, I would like to know if Opti-Blot Blue can be used instead of traditional coomassie with any Gel (such as TGX gels from Bio-Rad)?

    Thank you for contacting us. OptiBlot Blue is a ready-to-use Coomassie stain that is optimal for obtaining well defined protein bands in a single step. It can be replaced by traditional Coomassie, and be used with the any electrophoretic gel. I hope this ...

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