Optiblot DTT Reducer (10X) (ab119199)

Overview

  • Product nameOptiblot DTT Reducer (10X)
    See all Western blot buffer reagents
  • General notesOptiblot DTT Reducer should be added during sample preparation if you wish to perform reduced SDS-PAGE and reduce the disulfide bonds of proteins.

    Optiblot gels must be used with Optiblot buffers.

    Buffer recipes are available in the protocol tab.

Properties

    Protocols

    References for Optiblot DTT Reducer (10X) (ab119199)

    ab119199 has not yet been referenced specifically in any publications.

    Product Wall

    The stock solution of the Optiblot DTT Reducer (10X) ab119199 in 2M. This means that once diluted 10 times this produces a 200 mM solution. Therefore if you usually use 10 mM with your samples I would suggest diluting this 200 times. The higher concent...

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    I now have further information in regards to the storage of the Optiblot DTT Reducer (ab119199). I can confirm that this should be fine andshould remain stable for longer if stored at -20°C. I would recommend dividing the 1 mL into smaller aliquots...

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    Thank you for your reply.

    I have not seen that we have tested or have any additional data regarding the dimerization of this protein or which reducing agents might be more effective. We generally use a 2x Laemmli buffer with 4% SDS, or use o...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"