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Optiblot DTT Reducer (10X) (ab119199)

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Overview

  • Product nameOptiblot DTT Reducer (10X)See all Western blot buffer reagents ...
  • General notesOptiblot DTT Reducer should be added during sample preparation if you wish to perform reduced SDS-PAGE and reduce the disulfide bonds of proteins.

    Optiblot gels must be used with Optiblot buffers.

    Buffer recipes are available in the protocol tab.

    • Properties

  • Storage instructionsStore at -20°C.
  • Storage bufferConstituent: 31% DTT
  • Concentration information loading...
  • Research Areas

    • Protocols

    References for Optiblot DTT Reducer (10X) (ab119199)

    ab119199 has not yet been referenced specifically in any publications.

    Publishing research using ab119199 ? Please let us know so that we can cite the reference in this datasheet

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    What is the concentration of the Optiblot DTT Reducer (10x) ab119199

    The stock solution of the Optiblot DTT Reducer (10X) ab119199 in 2M. This means that once diluted 10 times this produces a 200 mM solution. Therefore if you usually use 10 mM with your samples I would suggest diluting this 200 times. The higher concentrat...

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    Hi,
    Regarding the DTT, I actually need them urgently, as I am planning to start immunoblotting from coming Monday. Since, I am currently working on a manuscript, it will be slightly problematic to get them later. Also, can we not store the unused DTT...

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    I now have further information in regards to the storage of the Optiblot DTT Reducer (ab119199). I can confirm that this should be fine andshould remain stable for longer if stored at -20°C. I would recommend dividing the 1 mL into smaller aliquots an...

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    I tested four samples and one control (HeLa RIPA lysate). Loading buffer has DTT (100mM final) and SDS (2% final). Samples are boiled. I guess I was wondering whether you guys have tested the dimerization of MCTP1 (if that's not possible, wh...

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    Thank you for your reply.

    I have not seen that we have tested or have any additional data regarding the dimerization of this protein or which reducing agents might be more effective. We generally use a 2x Laemmli buffer with 4% SDS, or use our ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"