Overview
- Product nameOptiblot ECL Max Detect Kit (4.6pg-4.7ng)See all ECL Substrate kits ...
- Product overview
Optiblot ECL Max Detect Kit (ab133408) is a new horseradish peroxidase (HRP) substrate specially developed to take advantage of the large linear dynamic range provided by CCD imaging.
Optiblot ECL Max Detect Kit produces a strong, extremely long-lived signal, which, combined with very low background levels, allows for long exposure times enabling the detection of low-abundance proteins. Additionally, the signal from Optiblot ECL Max Detect Kit is linear with respect to protein amount over a broad range of concentrations, displaying no substrate depletion at high protein loads, allowing the user to take full advantage of the linear range of the CCD detection method.
Optiblot ECL Max Detect Kit is also compatible with X-ray film detection, though the limited dynamic range of film will make resulting data less quantitative.
100ml kit for 1000cm2 membrane
200ml kit for 2000cm2 membrane
- Notes
Detection ranges stated should be used for guidance only as detection range is dependent on the molecular weight of a protein.
Recommended primary antibody dilution range: 1/5000 - 1/20,000
Recommended secondary antibody dilution range: 1/20,000 - 1/100,000
*Due to sensitivity of substrate, optimisation may be required:
Primary antibody concentration: start with usual primary antibody dilution and dilute 5 to 10 fold to find optimal conditions.
- Tested applicationsWB more details
Properties
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Components 200 ml 100 ml 20 ml Luminol/enhancer solution 1 x 100ml 1 x 50ml 1 x 10ml Peroxide Chemiluminescent Detection Reagent 1 x 100ml 1 x 50ml 1 x 10ml - Research Areas
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Alternative names
- ECLECL substrate
Applications
Our Abpromise guarantee covers the use of ab133408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB |
Optiblot ECL Max Detect Kit (4.6pg-4.7ng) images
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Western Blot 1/50000 primary antibody, 1/5000 secondary antibody, 10 second exposure -
Western Blot 1/25000 primary antibody, 1/5000 secondary antibody, 10 second exposureEach lane contains the following amount of COX2 recombinant protein (ab58868): 1) 100ng 2) 50ng 3) 25ng 4) 12.5ng 5) 6.25ng 6) 3.13ng 7) 1.56ng 8) 780pg 9) 390pg 10) 195pg 11) 98pg. The blot was probed with rabbit anti-COX2 antibody (ab15191) and with a rabbit secondary (ab97080). The blot was developed with 20ml of Optiblot ECL Max Detect.
Protocols
References for Optiblot ECL Max Detect Kit (4.6pg-4.7ng) (ab133408)
ab133408 has not yet been referenced specifically in any publications.
