• Product name
    Optiblot ECL Max Detect Kit (4.6pg-4.7ng)
    See all ECL Substrate kits
  • Product overview

    Optiblot ECL Max Detect Kit (ab133408) is a new horseradish peroxidase (HRP) substrate specially developed to take advantage of the large linear dynamic range provided by CCD imaging.

    Optiblot ECL Max Detect Kit produces a strong, extremely long-lived signal, which, combined with very low background levels, allows for long exposure times enabling the detection of low-abundance proteins. Additionally, the signal from Optiblot ECL Max Detect Kit is linear with respect to protein amount over a broad range of concentrations, displaying no substrate depletion at high protein loads, allowing the user to take full advantage of the linear range of the CCD detection method.

    Optiblot ECL Max Detect Kit is also compatible with X-ray film detection, though the limited dynamic range of film will make resulting data less quantitative.

    100ml kit for 1000cm2 membrane
    200ml kit for 2000cm2 membrane

  • Notes

    Detection ranges stated should be used for guidance only as detection range is dependent on the molecular weight of a protein.

    Recommended primary antibody dilution range: 1/5000 - 1/20,000
    Recommended secondary antibody dilution range: 1/20,000 - 1/100,000

    *Due to sensitivity of substrate, optimisation may be required:
    Primary antibody concentration: start with usual primary antibody dilution and dilute 5 to 10 fold to find optimal conditions.

  • Tested applications
    Suitable for: WBmore details



Our Abpromise guarantee covers the use of ab133408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.


  • Each lane contains the following amount of COX2 recombinant protein (ab58868): 1) 100ng 2) 50ng 3) 25ng 4) 12.5ng 5) 6.25ng 6) 3.13ng 7) 1.56ng 8) 780pg 9) 390pg 10) 195pg 11) 98pg. The blot was probed with rabbit anti-COX2 antibody (ab15191) and with a rabbit secondary (ab97080). The blot was developed with 20ml of Optiblot ECL Max Detect.



This product has been referenced in:
  • Wege S & Poirier Y Expression of the mammalian Xenotropic Polytropic Virus Receptor 1 (XPR1) in tobacco leaves leads to phosphate export. FEBS Lett 588:482-9 (2014). Read more (PubMed: 24374333) »

See 1 Publication for this product

Customer reviews and Q&As

ab133409 has been used for ELISA applications.

However, since there are many different readers, plates, etc., testing/evaluation is necessary to assess utility for your specific experimental conditions.

Thank you for contacting us.

We recommend developing, i.e. incubation with the substrate for 2 to 5 minutes. Longer incubation does not improve the performance, but it does not harm either in most cases. If the amount of protein detected is ...

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The product comparable to Super Signal west Dura is Optiblot ECL Max


Product Optiblot ECL Ultra ab133409 is even more sensi...

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ab133408 is compatible, however it would be better than Amersham Prime.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your inquiry.

Since you need a suitable ECL for imagers, I suggest to use ab133408 (Optiblot ECL Max Detect Kit (4.6pg-4.7ng)) which also corresponds with the ECL plus you are using at the moment.

ab133408 has been val...

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