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Optiblot ECL Max Detect Kit (ab133408) is a new horseradish peroxidase (HRP) substrate specially developed to take advantage of the large linear dynamic range provided by CCD imaging.
Optiblot ECL Max Detect Kit produces a strong, extremely long-lived signal, which, combined with very low background levels, allows for long exposure times enabling the detection of low-abundance proteins. Additionally, the signal from Optiblot ECL Max Detect Kit is linear with respect to protein amount over a broad range of concentrations, displaying no substrate depletion at high protein loads, allowing the user to take full advantage of the linear range of the CCD detection method.
Optiblot ECL Max Detect Kit is also compatible with X-ray film detection, though the limited dynamic range of film will make resulting data less quantitative.
100ml kit for 1000cm2 membrane
200ml kit for 2000cm2 membrane
Detection ranges stated should be used for guidance only as detection range is dependent on the molecular weight of a protein.
Recommended primary antibody dilution range: 1/5000 - 1/20,000
Recommended secondary antibody dilution range: 1/20,000 - 1/100,000
*Due to sensitivity of substrate, optimisation may be required:
Primary antibody concentration: start with usual primary antibody dilution and dilute 5 to 10 fold to find optimal conditions.
|Components||200 ml||100 ml||20 ml|
|Luminol/enhancer solution||1 x 100ml||1 x 50ml||1 x 10ml|
|Peroxide Chemiluminescent Detection Reagent||1 x 100ml||1 x 50ml||1 x 10ml|
Our Abpromise guarantee covers the use of ab133408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|