• Product name
    Optiblot ECL Ultra Detect Kit (1.2pg-2ng)
    See all ECL Substrate kits
  • Product overview

    Optiblot ECL Ultra Detect Kit (ab133409) is a horseradish peroxidase (HRP) substrate specially developed for Western blotting detection of very low abundance proteins. Optiblot ECL Ultra produces a strong, long-lived signal, which, combined with very low background levels, allows for long exposure times enabling the detection of low-abundance proteins. Additionally, the signal from Optiblot ECL Ultra is linear with respect to protein amount over a broad range of concentrations, allowing the user to accurately quantify protein bands.


    Optiblot ECL Ultra Detect Kit is most suitable for detection of low-abundance proteins or in situations when amounts of available primary antibodies are very limited and high dilution factors are desired, or when primary antibodies have relatively low binding constants.


    Optiblot ECL Ultra Detect Kit is also compatible with X-ray film detection, though the limited dynamic range of film will make resulting data less quantitative. Optiblot ECL Ultra Detect Kit also produces a chemifluorescent signal that can be detected with a fluorescence imaging system using appropriate excitation and emission settings.

    100ml kit for 1000cm2 membrane

    200ml kit for 2000cm2 membrane

  • Notes

    Detection ranges stated should be used for guidance only as detection range is dependent on the molecular weight of a protein.


    Recommended primary antibody dilution range: 1/5000 - 1/20,000

    Recommended secondary antibody dilution range: 1/20,000 - 1/100,000


    *Due to sensitivity of substrate, optimisation may be required:

    Primary antibody concentration: start with usual primary antibody dilution and dilute 5 to 10 fold to find optimal conditions.

  • Tested applications
    Suitable for: WBmore details



Our Abpromise guarantee covers the use of ab133409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.


  • Each lane contains the following amount of COX2 recombinant protein (ab58868): 1) 100ng 2) 50ng 3) 25ng 4) 12.5ng 5) 6.25ng 6) 3.13ng 7) 1.56ng 8) 780pg 9) 390pg 10) 195pg 11) 98pg. The blot was probed with rabbit anti-COX2 antibody (ab15191) and with a rabbit secondary (ab97080). The blot was developed with 20ml of Optiblot ECL Ultra Detect.



This product has been referenced in:
  • Kim GS  et al. Mutagenesis Study Reveals the Rim of Catalytic Entry Site of HDAC4 and -5 as the Major Binding Surface of SMRT Corepressor. PLoS One 10:e0132680 (2015). WB . Read more (PubMed: 26161557) »

See 1 Publication for this product

Customer reviews and Q&As

ab133409 has been used for ELISA applications.

However, since there are many different readers, plates, etc., testing/evaluation is necessary to assess utility for your specific experimental conditions.

Since you need a suitable ECL for imagers, I suggest to use ab133408 (Optiblot ECL Max Detect Kit (4.6pg-4.7ng)) which also corresponds with the ECL plus you are using at the moment.

ab133408 has been validated for CCD/laser use and generates...

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