Optiblot ECL Ultra Detect Kit (1.2pg-2ng) (ab133409)

Overview

• Product nameOptiblot ECL Ultra Detect Kit (1.2pg-2ng)See all ECL Substrate kits ...
• Product overview

  Optiblot ECL Ultra Detect Kit (ab133409) is a horseradish peroxidase (HRP) substrate specially developed for Western blotting detection of very low abundance proteins. Optiblot ECL Ultra produces a strong, long-lived signal, which, combined with very low background levels, allows for long exposure times enabling the detection of low-abundance proteins. Additionally, the signal from Optiblot ECL Ultra is linear with respect to protein amount over a broad range of concentrations, allowing the user to accurately quantify protein bands.

   

  Optiblot ECL Ultra Detect Kit is most suitable for detection of low-abundance proteins or in situations when amounts of available primary antibodies are very limited and high dilution factors are desired, or when primary antibodies have relatively low binding constants.

   

  Optiblot ECL Ultra Detect Kit is also compatible with X-ray film detection, though the limited dynamic range of film will make resulting data less quantitative. Optiblot ECL Ultra Detect Kit also produces a chemifluorescent signal that can be detected with a fluorescence imaging system using appropriate excitation and emission settings.

  100ml kit for 1000cm² membrane

  200ml kit for 2000cm² membrane

• Notes

  Detection ranges stated should be used for guidance only as detection range is dependent on the molecular weight of a protein.

   

  Recommended primary antibody dilution range: 1/5000 - 1/20,000

  Recommended secondary antibody dilution range: 1/20,000 - 1/100,000

   

  *Due to sensitivity of substrate, optimisation may be required:

  Primary antibody concentration: start with usual primary antibody dilution and dilute 5 to 10 fold to find optimal conditions.

• Tested applicationsWB more details

Properties

• Components	200 ml	100 ml	20 ml
  Luminol/enhancer solution	1 x 100ml	1 x 50ml	1 x 10ml
  Peroxide Chemiluminescent Detection Reagent	1 x 100ml	1 x 50ml	1 x 10ml

• Research Areas
  • Kits/ Lysates/ Other
  • Tools and Reagents
  • Western Blot Tools and Reagents
  • ECL kits

Applications

Optiblot ECL Ultra Detect Kit (1.2pg-2ng) images

• 

  Western Blot 1/50000 primary antibody, 1/5000 secondary antibody, 10 second exposure
• 

  Western Blot 1/25000 primary antibody, 1/5000 secondary antibody, 10 second exposure
  Each lane contains the following amount of COX2 recombinant protein (ab58868): 1) 100ng 2) 50ng 3) 25ng 4) 12.5ng 5) 6.25ng 6) 3.13ng 7) 1.56ng 8) 780pg 9) 390pg 10) 195pg 11) 98pg. The blot was probed with rabbit anti-COX2 antibody (ab15191) and with a rabbit secondary (ab97080). The blot was developed with 20ml of Optiblot ECL Ultra Detect.

Protocols

Protocol Booklet