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Optiblot ECL Ultra Detect Kit (ab133409) is a horseradish peroxidase (HRP) substrate specially developed for Western blotting detection of very low abundance proteins. Optiblot ECL Ultra produces a strong, long-lived signal, which, combined with very low background levels, allows for long exposure times enabling the detection of low-abundance proteins. Additionally, the signal from Optiblot ECL Ultra is linear with respect to protein amount over a broad range of concentrations, allowing the user to accurately quantify protein bands.
Optiblot ECL Ultra Detect Kit is most suitable for detection of low-abundance proteins or in situations when amounts of available primary antibodies are very limited and high dilution factors are desired, or when primary antibodies have relatively low binding constants.
Optiblot ECL Ultra Detect Kit is also compatible with X-ray film detection, though the limited dynamic range of film will make resulting data less quantitative. Optiblot ECL Ultra Detect Kit also produces a chemifluorescent signal that can be detected with a fluorescence imaging system using appropriate excitation and emission settings.
100ml kit for 1000cm2 membrane
200ml kit for 2000cm2 membrane
Detection ranges stated should be used for guidance only as detection range is dependent on the molecular weight of a protein.
Recommended primary antibody dilution range: 1/5000 - 1/20,000
Recommended secondary antibody dilution range: 1/20,000 - 1/100,000
*Due to sensitivity of substrate, optimisation may be required:
Primary antibody concentration: start with usual primary antibody dilution and dilute 5 to 10 fold to find optimal conditions.
|Components||200 ml||100 ml||20 ml|
|Luminol/enhancer solution||1 x 100ml||1 x 50ml||1 x 10ml|
|Peroxide Chemiluminescent Detection Reagent||1 x 100ml||1 x 50ml||1 x 10ml|
Our Abpromise guarantee covers the use of ab133409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|