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Optiblot Fluorescent Western Blot Kit allows the assay of two proteins at once, increasing the quality and quantity of information that can be gained from a single blot. Assay a loading control alongside a protein of interest. Assay phosphorylated and non-phosphorylated isoforms of a protein simultaneously. The Optiblot Fluorescent Western Blot Kit protocol saves time and money since there is no need to strip and re-probe a blot, no use of disposable film, and the blot can be imaged immediately, without drying.
The fluorescent dyes provided with the Optiblot Fluorescent Western Blot Kit outperform Cy dyes and allow detection of low pg amounts of protein.
Compatible with CCD imagers equipped with Cy3(Green)/Cy5(Red) excitation and emission. (e.g. ChemiDoc MP, LAS 4000, Fluorchem Q and M systems, G:BOX, Gel Logic, Fusion FX5, Typhoon imager, etc)
|APC-goat-anti-rabbit IgG antibody||1 x 50µl|
|Background Quenching Sheets||10 units|
|Immunoblot washing solution, 10x concentrate||1 x 120ml|
|ab133411 - Low-fluorescence PVDF transfer membrane 9x7 cm||10 units|
|Protein-free blocking solution, 5x concentrate||1 x 50ml|
|RPE-goat-anti-mouse IgG antibody||1 x 50µl|
Our Abpromise guarantee covers the use of ab133410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
ab133410 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"