Overview
- Product nameOptiblot Fluorescent Western Blot Kit
- Tests1 x 10 assay
- Product overview
Optiblot Fluorescent Western Blot Kit allows the assay of two proteins at once, increasing the quality and quantity of information that can be gained from a single blot. Assay a loading control alongside a protein of interest. Assay phosphorylated and non-phosphorylated isoforms of a protein simultaneously. The Optiblot Fluorescent Western Blot Kit protocol saves time and money since there is no need to strip and re-probe a blot, no use of disposable film, and the blot can be imaged immediately, without drying.
The fluorescent dyes provided with the Optiblot Fluorescent Western Blot Kit outperform Cy dyes and allow detection of low pg amounts of protein.
Compatible with CCD imagers equipped with Cy3(Green)/Cy5(Red) excitation and emission. (e.g. ChemiDoc MP, LAS 4000, Fluorchem Q and M systems, G:BOX, Gel Logic, Fusion FX5, Typhoon imager, etc)
- Tested applicationsWB more details
Properties
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Components 10 tests APC-goat-anti-rabbit IgG antibody 1 x 50µl Background Quenching Sheets 10 units Immunoblot washing solution, 10x concentrate 1 x 120ml ab133411 - Low-fluorescence PVDF transfer membrane 9x7 cm 10 units Protein-free blocking solution, 5x concentrate 1 x 50ml RPE-goat-anti-mouse IgG antibody 1 x 50µl - Research Areas
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Alternative names
- fluorescent western blotting kitWestern blot kit
Applications
Our Abpromise guarantee covers the use of ab133410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB |
Optiblot Fluorescent Western Blot Kit images
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Western blot - Obtiblot Fluorescent Western Blot Kit (ab133410)Optiblot Conjugates provide a brighter signal than the ECL Plex Western Blotting system. Duplicate Western blots containing samples of AFP and CEA proteins were treated identically and probed with the same mixture of mouse anti-AFP and rabbit anti-CEA primary antibodies. One blot was then stained with Optiblot conjugates (a) and the other with and identical concentration of Cy3 anti-mouse and Cy5 anti-rabbit antibodies (b). Under identical imaging conditions, Optiblot provides a brighter signal, and 10x greater sensitivity. -
Western blot - Optiblot Fluorescent Western Blot Kit (ab133410)Simultaneous detection of EGFR and phospho-EGFR with Obtiblot Fluorescent Western Blot Kit. The increase in phosphorylation of EGFR in response to EGF was detected. Lysates from A431 cells (lane 1) and from A431 cells treated with EGF (lane 2) were blotted and EGFR detected in the green channel (b) or phospho-EGFR detected in the red channel (c). The two channels are superimposed in (a). Lane 3: molecular weight markers.
Protocols
References for Optiblot Fluorescent Western Blot Kit (ab133410)
ab133410 has not yet been referenced specifically in any publications.
