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Optiblot LDS Sample Buffer (4X) (ab119196)

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Overview

  • Product nameOptiblot LDS Sample Buffer (4X)See all Western blot buffer reagents ...
  • Tested applicationsSDS-PAGE, WB more details
  • General notesOptiblot LDS Sample Buffer should be used to prepare all samples for runing on Optiblot Precast Gels. Specifically formulated for the Optiblot buffer system to provide the best running conditions and results.

    Optiblot gels must be used with Optiblot buffers

    Buffer recipes are available in the protocol tab.

    • Properties

  • Storage instructionsStore at +4°C.
  • Storage bufferpH: 7.75
    Constituents: 12% Triethanolamine, 40% Glycerol, 9.3% Hydrochloric acid, 0.07% EDTA, 0.025% Phenol red, 4% Polysucrose 400, 4% Lithium dodecyl sulphate, 0.025% Coomassie Brilliant Blue
    Note: The pH should be between 7.7 and 7.8 @ 25°C.
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab119196 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent concentration.
    WB WB: Use at an assay dependent concentration.

    References for Optiblot LDS Sample Buffer (4X) (ab119196)

    ab119196 has not yet been referenced specifically in any publications.

    Publishing research using ab119196 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab119196 Submit a Question for ab119196

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    Thank you for your prompt reply. I do have one more clarification.
    I do not have the chemicals required for preparing Optiblot LDS sample buffer. So can I use the Laemli sample buffer instead? It contains tris HCl buffer pH 6.8, 2% SDS, mercaptoethan...

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    Thank you for your reply.
    I'd suggest using the sample buffer that is compatible with the gel, namely ab119196.
    http://www.abcam.com/optiblot-lds-sample-buffer-4x-ab119196.html
    This is what is recommended to be used.
    I can doublecheck ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"