Optiblot Prestained Marker (ab119210)

Overview

  • Product nameOptiblot Prestained MarkerSee all Prestained Protein Ladder reagents ...
  • Tested applicationsWB, SDS-PAGEmore details
  • General notesOptiblot Prestained Markers have been specifically formulated for use with Optiblot Gels and Buffers. Marker proteins are conjugated to dye molecules to show position before staining indicating how far your protein has migrated and to confirm the blotting efficiency without the need to stain your target protein.

    Optiblot Prestained Markers consist of 195, 142, 96, 71, 48, 33, 28, 22, 12 and 7.6 kDa standards. For additional convenience and ease of identification, the 71 and 28 kDa standards have been conjugated to an orange dye molecule.

    A load of only 4 µl Optiblot Pre-stained Markers is sufficient for a high intensity marker ladder (nearly 2.5x less loading than conventional ladders).

    The prestained markers are provided ready to use in 1x Optiblot sample buffer(ab119196). Recommended volumes to load for a 12 well comb are:

    To Monitor during run: 10 µl/well
    Visisble after blotting: 4 µl/well
    Visible after staining: 4 µl/well

    Optiblot gels must be used with Optiblot buffers.

    Buffer recipes are available in the protocol tab.

Properties

    Applications

    Our Abpromise guarantee covers the use of ab119210 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent concentration.
    SDS-PAGE Use at an assay dependent concentration.

    Optiblot Prestained Marker images

    • ab119210 separated on a 12%, 12 well Optiblot gel.

      Lane 1: Prior to staining with Optiblot blue (ab119211)
      Lane 2: After staining with Optiblot blue (ab119211)

    References for Optiblot Prestained Marker (ab119210)

    ab119210 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your patience. My colleague in the lab informed me that our optiblot range of protein molecular weight markers do contain His-tagged proteins. The markers ab115832 and ab48854 do not contain His-tagged proteins. I hope this helps, please ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"