Optiblot Reducing SDS Run Buffer (20X) (ab119195)

Overview

  • Product nameOptiblot Reducing SDS Run Buffer (20X)
    See all Western blot buffer reagents
  • General notesOptiblot Reducing SDS Run Buffer has been specifically designed and formulated for use with Optiblot gels to provide the best running conditions and optimal sample resolution. Based on a Tris-MOPS buffer system this run buffer provides excellent band resolution and fast run time. A 10% Optiblot gel will be run to completion within 35 minutes. Separation pattern is similar to that produced by a conventional MES buffer system.

    Optiblot gels must be used with Optiblot buffers

    Buffer recipes are available in the protocol tab.

Properties

    Protocols

    References for Optiblot Reducing SDS Run Buffer (20X) (ab119195)

    ab119195 has not yet been referenced specifically in any publications.

    Product Wall

    Quick and easy to use without messy preparation

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    We opted to use the reducing SDS run buffer in conjunction with the Optiblot WB kits. The main driver for this was cost and the ease of use/preparation. The buffers are diluted prior to use and work very well for our applications in standard WB techniques. When used in conjunction with Optiblot gels the results are excellent with clearly defined bands. We're very pleased with the Optiblot range as it has significantly increased our throughput.

    We used a typical protocol:

    Protein was extracted using RIPA buffer extraction, followed by treatment at 37 degrees Celsius for 30 minutes in Optiblot LDS Sample Buffer (4X). 100ug was loaded per well and run for 45 minutes at 180V. Following transfer and staining we see nice clearly defined bands for our samples.
    Username

    Dr. Raj Badhan

    Verified customer

    Submitted Aug 27 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"