Overview

  • Product name
    Optiblot SDS-PAGE Sample Preparation Kit
  • Product overview

    The Optiblot SDS-PAGE Sample Preparation Kit (ab133414) provides a means to quickly concentrate protein samples, and separate them from buffers that interfere with electrophoresis.

    The fast, efficient protocol generates samples ready to load on a gel in less than ten minutes, much more quickly than can be achieved with alternate methods such as dialysis, or acetone or TCA precipitation. Additionally, the Optiblot SDS-PAGE Sample Preparation protocol is easily scaled up, allowing multiple samples to be prepared in parallel.

  • Tested applications
    Suitable for: Purificationmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab133414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Purification Use at an assay dependent concentration.

Images

  • Resin concentration is compatible with Western blot detection. Western blots were created with samples of A431 cell extract (panels a and b, lane 2), diluted cell extract (lane 3) and the diluted extract concentrated using the Optiblot SDS-PAGE Sample Preparation Kit protocol (lane 4). The blots were detected using the Optiblot Fluorescent Western Blot Kit (ab133410). No bands were detectable in the diluted cell extract when stained for either GAPDH (panel a, lane 3) or SRC (panel b, lane 3). However, after resin concentration, bands are easily visualized for both proteins (lanes 4). Band intensities are indicated.

Protocols

References

ab133414 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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