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The Optiblot SDS-PAGE Sample Preparation Kit (ab133414) provides a means to quickly concentrate protein samples, and separate them from buffers that interfere with electrophoresis.
The fast, efficient protocol generates samples ready to load on a gel in less than ten minutes, much more quickly than can be achieved with alternate methods such as dialysis, or acetone or TCA precipitation. Additionally, the Optiblot SDS-PAGE Sample Preparation protocol is easily scaled up, allowing multiple samples to be prepared in parallel.
|Components||10 tests||25 tests|
|Extraction loading buffer||1 x 1ml||1 x 1ml|
|Protein filters||10 units||25 units|
|Resin||1 x 500ml||2 x 500ml|
Our Abpromise guarantee covers the use of ab133414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Purification||Use at an assay dependent concentration.|
ab133414 has not yet been referenced specifically in any publications.