Overview

  • Product name
    Optiblot SDS Run Buffer (20X)
    See all Western blot buffer reagents
  • Tested applications
    Suitable for: SDS-PAGEmore details
  • General notes
    Optiblot SDS RunBuffer is optimised for use with Optiblot Precast Gels to provide the best running conditions and optimal sample resolution. Supplied in two volumes, 500ml or a in convenient 5 litre cube for high volume users. Based on a Tris-Tricine buffer system this gives improved resolution at lower molecular weights whilst retaining excellent resolution at the high moleculr weights. Separation pattern is similar to that produced by a conventional MOPS buffer system.

    Optiblot gels must be used with Optiblot buffers.

    Buffer recipes are available in the protocol tab.

Properties

  • Storage instructions
    Store at room temperature.
  • Storage buffer
    pH: 8.30
    Constituents: 14% Tricine, 15% Tris, 0.95% Sodium bisulfite, 2% SDS
    Note: The pH should be between 8.2 and 8.3 @ 25°C. For Non-reduced samples (especially antibodies), omit the Sodium Bisulfite. For best results use low conductance ingredients to formulate the buffers. Do NOT use acid or base to adjust pH of buffers.
  • Concentration information loading...
  • Research areas
  • Alternative names
    • SDS PAGE Western blot buffer
    • WB buffer
    • Western Blot

Applications

Our Abpromise guarantee covers the use of ab119197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
SDS-PAGE Use at an assay dependent dilution.

Protocols

References

ab119197 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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