Optiblot SDS Run Buffer (20X) (ab119197)
Optiblot gels must be used with Optiblot buffers.
Buffer recipes are available in the protocol tab.
Constituents: 14% Tricine, 15% Tris, 0.95% Sodium bisulfite, 2% SDS
Note: The pH should be between 8.2 and 8.3 @ 25°C. For Non-reduced samples (especially antibodies), omit the Sodium Bisulfite. For best results use low conductance ingredients to formulate the buffers. Do NOT use acid or base to adjust pH of buffers.
Our Abpromise guarantee covers the use of ab119197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|SDS-PAGE||SDS-PAGE: Use at an assay dependent dilution.|
References for Optiblot SDS Run Buffer (20X) (ab119197)
ab119197 has not yet been referenced specifically in any publications.