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Optiblot TGS Blot Stock Buffer (10X/20X) (ab119198)

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Overview

  • Product nameOptiblot TGS Blot Stock Buffer (10X/20X)See all Western blot buffer reagents ...
  • Tested applicationsWB more details
  • General notesOptiblot TGS Blot Buffer contains 0.25M Tris (base), 1.92M Glycine, and 1% SDS. Optiblot TGS Blot Buffer should be used diluted to equilibrate gels prior to blotting.10x for use in semi-dry blotters and 20x for use in tank blotters.

    Optiblot gels must be used with Optiblot buffers

    Buffer recipes are available in the protocol tab.

    • Properties

  • Storage instructionsStore at +4°C.
  • Storage bufferpH: 8.55
    Constituents: 5% Glycine, 4% Tris, 0.5% Sodium bisulfite, 1% SDS
    Note: The pH should be between 8.5 and 8.6 @ 25°C. For best results use low conductance ingredients to formulate the buffers. Do NOT use acid or base to adjust pH of buffers.
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab119198 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: 1/10 - 1/20. Dependent on application

    References for Optiblot TGS Blot Stock Buffer (10X/20X) (ab119198)

    ab119198 has not yet been referenced specifically in any publications.

    Publishing research using ab119198 ? Please let us know so that we can cite the reference in this datasheet

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