Overview

  • Product name
    Anti-Ornithine Decarboxylase antibody
    See all Ornithine Decarboxylase primary antibodies
  • Description
    Rabbit polyclonal to Ornithine Decarboxylase
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 180 and 433 of Human Ornithine Decarboxylase (NP_002530).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, and Raji lysates. Molt-4 whole cell lysate. IF/ICC: HeLa cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab97395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 51 kDa.

Target

Images

  • ICC/IF image of ab97395 stained HeLa cells. The cells were 4% Formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97395, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Ornithine Decarboxylase antibody (ab97395) at 1/1000 dilution + Molt-4 whole cell lysate at 30 µg

    Predicted band size : 51 kDa
    10% SDS PAGE

References

This product has been referenced in:
  • Wang X  et al. Arginine decarboxylase and agmatinase: an alternative pathway for de novo biosynthesis of polyamines for development of mammalian conceptuses. Biol Reprod 90:84 (2014). Read more (PubMed: 24648395) »
  • Wang X  et al. Functional role of arginine during the peri-implantation period of pregnancy. II. Consequences of loss of function of nitric oxide synthase NOS3 mRNA in ovine conceptus trophectoderm. Biol Reprod 91:59 (2014). Read more (PubMed: 25061098) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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