Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Proteins and Peptides
- Secondary antibodies
Buying a fast track?
We are able to bring you this product at a low price as it is not yet fully characterized. Our Scientific Support team are available to assist you, but we cannot offer refunds or replacements on this product.
What is a fast track? »Overview
Product name
Anti-Osteocrin antibody
See all Osteocrin products (2) ...
Description
Rabbit polyclonal to Osteocrin
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
Cross reactivity
Predicted to work with
Mouse, Rat, Sheep, Chicken, Cow, Human, Pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Osteocrin.
(Peptide available as ab409 04.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Stem Cells >> Mesenchymal Stem Cells >> Secreted Molecules
-
Western blot - Osteocrin antibody (ab40905)
(enlarge)
Applications
Application notes
This antibody gave a positive result in ELISA against the immunizing peptide (ab40904).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Appears to modulate osteoblastic differentiation. Could also function as an autocrine and paracrine factor linked to glucose metabolism in skeletal muscle.
Tissue specificity
Expressed in bone.
Cellular localization
Secreted.
Target information above from: UniProt accessionP61366
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Musclin antibody
- Osteocrin antibody
- OSTN antibody
- OSTN_HUMAN antibody
Database links
- Entrez Gene: 424907 Chicken
- Entrez Gene: 344901 Human
- Entrez Gene: 239790 Mouse
- Entrez Gene: 360730 Rat
- Omim: 610280 Human
- SwissProt: P61366 Human
- SwissProt: Q149W1 Mouse
- SwissProt: P61364 Mouse
see all
Anti-Osteocrin antibody images:
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Western blot - Osteocrin antibody (ab40905)
All lanes : Anti-Osteocrin antibody (ab40905) at 1 µg/ml
Lane 1 :
Lane 2 : Liver (Rat) Tissue Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 :
Lane 6 :
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 15 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Osteocrin has previously been shown to run at 5 & 11 kDa, with the MW of the unprocessed precursor being 15 kDa. We are unsure as to why ab49405 is giving a MW of 50 kDa. For Rat, however, SwissProt suggests that expression of Osteocrin is almost exclusively seen in Skeletal Muscle. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
References for Anti-Osteocrin antibody (ab40905)
ab40905 has not yet been referenced specifically in any publications.
Publishing research using ab40905? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"