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Overview

  • Product nameOsteopontin Human ELISA KitSee all Osteopontin kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 50 pg/ml
  • Range
    74.07 pg/ml - 18000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 103.5 95% - 118%
    Serum 89.92 88% - 108%
    Plasma 92.41 90% - 109%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100618 Human Osteopontin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Osteopontin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for human Osteopontin coated on a 96-well plate. Standards and samples are pipette into the wells and Osteopontin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Osteopontin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteopontin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsELISA more details

Properties

  • FunctionBinds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
    Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • Tissue specificityBone. Found in plasma.
  • Sequence similaritiesBelongs to the osteopontin family.
  • Post-translational
    modifications    Extensively phosphorylated by FAM20C in the extracelllular medium at multiple sites within the S-x-E/pS motif.
    N- and O-glycosylated.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P10451 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        BNSPBone sialoprotein 1Bone sialoprotein I
        BSPIEarly T lymphocyte activation 1 ETA 1MGC110940NephropontinOPNOsteopontinOSTP_HUMANSecreted phosphoprotein 1Secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1)Secreted phosphoprotein 1 (osteopontin bone sialoprotein)SPP-1SPP1Urinary stone proteinUropontin
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100618 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Osteopontin Human ELISA Kit images

      • ELISA - Osteopontin Human ELISA Kit (ab100618)
        ELISA - Osteopontin Human ELISA Kit (ab100618)

      Protocols

      References for Osteopontin Human ELISA Kit (ab100618)

      ab100618 has not yet been referenced specifically in any publications.

      Publishing research using ab100618 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100618 Submit a Question for ab100618

      Displaying 1 - 8 of 8 results for Abreviews and Q&A

      10
      Votes: Highest First

      Product code: 100618
      Inquiry: Hi, We are interested in purchasing the human Osteopontin ELISA, however we need additional Osteopontin protein to validate the assay. Which of the Osteopontin proteins available represents the one in the ELISA? Thanks

      Thank you for contacting us.
      Though we have not tested the protein ab81549 (http://www.abcam.com/osteopontin-protein-active-ab81549.html) with ab100618. However it is most likely to work.
      I hope this information is helpful to you. Please do not h...

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      Thank you so much for your company response to my question about Human Osteopontin ELISA kit ab100618). We done our test today and find dilution factor about 25 fold, so I thought it's maybe useful for your company as a scientific report.

      Best ...

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      Thank you for taking the time to contact me and let us know.

      I'm pleased to hear the results were successful and that you have found a suitable dilution factor for your samples.

      Good luck with your research, and please do not hesitat...

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      Hello

      We bought abcam human osteopontin ELISA kit (ab100618). Its protocol said 'you must determine optimal dilution factor'. We want to determine osteopontin level in human serums but can you help me to find this optimal dilution factor?
      ...

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      Thank you for your enquiry.

      I can confirm that the suggested dilution for normal serum/plasma when testing with this kit is 3-100 fold.

      Please note that this will depend on expression level in the samples you have and so this is a gu...

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      Dear Sir/Madam,

      Can you send us the quote for the above product? Do you have antibody specific for different isoforms for OPN or any otherrelated product? Do you also have ELISA kit for OPN?
      Thank you.

      --
      Kind regards,

      Thank youfor your inquiry.

      The quote for ab8448 has been send in a separate email.

      I am happy to confirm that we offer ELISA kits for Osteopontin:

      ab100618 (Osteopontin Human ELISA Kit)

      http://www.abcam.com/index.ht...

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      May I ask can this ELISA kit measure the MMP-cleaved or Thrombin-clearved osteopontin?

      Thank you for contacting us.

      We received the following explanation from the lab:

      The full length Osteopontin protein (aa 17-314) was used as the immunogen for the antibodies used in thekit ab100618. And as epitope mapping was not perf...

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      El mes pasado hice un pedido de 9 kits de ELISA de Abcam y ayer, al usar uno de ellos, comprobamos que uno de los reactivos estaba contaminado. Era el bote de 5XELISA Assay Diluent B (Item E) que tenía una especie de hongo gigantesco. Como ya ten&i...

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      Lamento mucho lo ocurrido. Es más que probable que los resultados obtenidos no sean correctos debido a la contaminación del buffer.

      Os he mandado un kit nuevo completamente gratuito para compensar. Recibiréis un mail autom&a...

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      I have used half of this kit and realized that I received a shortage of Item G.  I would like a replacement.

      Thank you for contacting Abcam. I am sorry that you did not receive the full amount of product and for the inconvenience this has caused. As requested, I have issued a free of charge replacement. To check the status of the order please contact our...

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      I have bought an Osteopontin human Elisa kit (ab100618). I would like to know if this kit can be used on cell culture medium thas has been frozen?

      Thank you for your patience. Here is the lab's response to your question: While testing fresh media is always ideal, frozen media is usually more practical and will certainly be compatible with our ELISA kits. I hope this information is helpful ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"