Osteopontin Mouse ELISA Kit (ab100734)
Overview
- Product nameOsteopontin Mouse ELISA KitSee all Osteopontin kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 4 pg/ml
- Range2.74 pg/ml - 2000 pg/ml
- Recovery
100 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 85.46 78% - 105% Serum 115.6 103% - 122% Plasma 110.4 87% - 130% - Species reactivityReacts with: Mouse
- Product overview
ab100734 Osteopontin (OPN) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Osteopontin in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Osteopontin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteopontin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Osteopontin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteopontin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 400X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent B 5x concentrated buffer. For detection antibody and HRP- Streptavidin diluent. 1 x 15ml Assay Diluent C diluent buffer . For Standard/Sample (serum/plasma samples/cell culture medium) diluent. 1 x 30ml Detection Antibody Osteopontin: biotinylated anti-mouse Osteopontin 2 vials OPN Standard Mouse (recombinant) 2 vials Osteopontin Microplate (12 strips x 8 wells) coated with anti-mouse Osteopontin 1 unit Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml -
Research Areas
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
modificationsExtensively phosphorylated by FAM20C in the extracelllular medium at multiple sites within the S-x-E/pS motif.
N- and O-glycosylated.
Target information above from: UniProt accession
P10451
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- BNSPBone sialoprotein 1Bone sialoprotein I
- BSPIEarly T lymphocyte activation 1 ETA 1MGC110940NephropontinOPNOsteopontinOSTP_HUMANSecreted phosphoprotein 1Secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1)Secreted phosphoprotein 1 (osteopontin bone sialoprotein)SPP-1SPP1Urinary stone proteinUropontin
see all
- Entrez Gene: 20750 Mouse
- SwissProt: P10923 Mouse
- Unigene: 288474 Mouse
Applications
Our Abpromise guarantee covers the use of ab100734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA |
Osteopontin Mouse ELISA Kit images
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ELISA - Osteopontin Mouse ELISA Kit (ab100734) -
ELISA - Osteopontin Mouse ELISA Kit - 1 x 96 Well Plate (ab100734)This image is courtesy of Anonymous Abreview.Osteopontin Mouse ELISA Kit - 1 x 96 Well Plate (ab100734) being used to detect Osteopontin in a 10 µg Mouse lung tissue homogenate sample. Recombinant mouse OPN of known concentration was used as positive control.
Protocols
References for Osteopontin Mouse ELISA Kit (ab100734)
This product has been referenced in:
- Fang X et al. Ameliorated ConA-induced hepatitis in the absence of PKC-theta. PLoS One 7:e31174 (2012). Mouse . Read more (PubMed: 22347449) »
